SCOTfluors: Small, Conjugatable, Orthogonal, and Tunable Fluorophores for In Vivo Imaging of Cell Metabolism

  • Angew Chem Int Ed Engl. 2019 May 20;58(21):6911-6915. doi: 10.1002/anie.201900465.
Sam Benson  1 ,  Antonio Fernandez  1 ,  Nicole D Barth  1 ,  Fabio de Moliner  1 ,  Mathew H Horrocks  2 ,  C Simon Herrington  3 ,  Jose Luis Abad  4  5 ,  Antonio Delgado  4  5 ,  Lisa Kelly  1 ,  Ziyuan Chang  1 ,  Yi Feng  1 ,  Miyako Nishiura  6 ,  Yuichiro Hori  6 ,  Kazuya Kikuchi  6 ,  Marc Vendrell  1
Affiliations
  • 1. Centre for Inflammation Research, The University of Edinburgh, EH16 4TJ, Edinburgh, UK.
  • 2. UK Dementia Research Institute and EaStCHEM School of Chemistry, The University of Edinburgh, EH9 3FJ, Edinburgh, UK.
  • 3. Edinburgh Cancer Research Centre, EH4 2XR, Edinburgh, UK.
  • 4. Research Unit on Bioactive Molecules, Institute for Advanced Chemistry of Catalonia, 08034, Barcelona, Spain.
  • 5. University of Barcelona, Faculty of Pharmacy, Unit of Pharmaceutical Chemistry (CSIC Associated Unit), Barcelona, Spain.
  • 6. Graduate School of Engineering, Osaka University, Suita, Japan.
PMID: 30924239 DOI: 10.1002/anie.201900465
Abstract

The transport and trafficking of metabolites are critical for the correct functioning of live cells. However, in situ metabolic imaging studies are hampered by the lack of fluorescent chemical structures that allow direct monitoring of small metabolites under physiological conditions with high spatial and temporal resolution. Herein, we describe SCOTfluors as novel small-sized multi-colored fluorophores for real-time tracking of essential metabolites in live cells and in vivo and for the acquisition of metabolic profiles from human Cancer cells of variable origin.

Keywords
cancer; fluorescence; imaging agents; metabolites; super-resolution imaging.
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