Astin C Production by the Endophytic Fungus Cyanodermella asteris in Planktonic and Immobilized Culture Conditions

  • Biotechnol J. 2019 Aug;14(8):e1800624. doi: 10.1002/biot.201800624.
Antoine Vassaux  1  2 ,  Cédric Tarayre  1 ,  Anthony Arguëlles-Arias  1 ,  Philippe Compère  3 ,  Frank Delvigne  1 ,  Patrick Fickers  1 ,  Linda Jahn  4 ,  Alexander Lang  5 ,  Valérie Leclère  2 ,  Jutta Ludwig-Müller  4 ,  Marc Ongena  1 ,  Thomas Schafhauser  6 ,  Samuel Telek  1 ,  Ariane Théatre  1 ,  Willem J H van Berkel  7 ,  Micheline Vandenbol  8 ,  Karl-Heinz van Pée  5 ,  Luc Willems  1  9 ,  Wolfgang Wohlleben  6 ,  Philippe Jacques  1
Affiliations
  • 1. TERRA Teaching and Research Centre, Microbial Processes and Interactions, Gembloux Agro-Bio Tech, University of Liege, Avenue de la Faculté d' Agronomie, B-5030, Gembloux, Belgium.
  • 2. EA 7394-ICV-Institut Charles Viollette, University Lille, INRA, ISA, University Artois, University Littoral Côte d'Opale, F-59000, Lille, France.
  • 3. Département de Biologie, Ecologie et Evolution et Cellule d'Appui à la Recherche et à l'Enseignement en Microscopie, Université de Liège, Allée du Six Ao û t 15, B-4000, Liege, Belgium.
  • 4. Institut für Botanik, Technische Universität Dresden, 01062, Dresden, Germany.
  • 5. Allgemeine Biochemie, Technische Universität Dresden, 01069, Dresden, Germany.
  • 6. Mikrobiologie Biotechnologie, Interfakultäres Institut für Mikrobiologie und Infektionsmedizin, Eberhard Karls Universität Tübingen, Auf der Morgenstelle 28, 72076, Tübingen, Germany.
  • 7. Laboratory of Biochemistry, Wageningen University & Research, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.
  • 8. TERRA Teaching and Research Centre, Microbiologie et Génomique, Gembloux Agro-Bio Tech, University of Liege, Avenue de la Faculté d'Agronomie, B-5030, Gembloux, Belgium.
  • 9. GIGA Research, Cellular and Molecular Epigenetics, University of Liege, Allée de l'Hôpital 1, B-4000, Liege, Belgium.
PMID: 31161690 DOI: 10.1002/biot.201800624
Abstract

The Fungal endophyte Cyanodermella asteris (C. asteris) has been recently isolated from the medicinal plant Aster tataricus (A. tataricus). This fungus produces astin C, a cyclic pentapeptide with Anticancer and anti-inflammatory properties. The production of this secondary metabolite is compared in immobilized and planktonic conditions. For immobilized cultures, a stainless steel packing immersed in the culture broth is used as a support. In these conditions, the fungus exclusively grows on the packing, which provides a considerable advantage for astin C recovery and purification. C. asteris metabolism is different according to the culture conditions in terms of substrate consumption rate, cell growth, and astin C production. Immobilized-cell cultures yield a 30% increase of astin C production, associated with a 39% increase in biomass. The inoculum type as spores rather than hyphae, and a pre-inoculation washing procedure with sodium hydroxide, turns out to be beneficial both for astin C production and fungus development onto the support. Finally, the influence of culture parameters such as pH and medium composition on astin C production is evaluated. With optimized culture conditions, astin C yield is further improved reaching a five times higher final specific yield compared to the value reported with astin C extraction from A. tataricus (0.89 mg g-1 and 0.16 mg g-1 respectively).

Keywords
Cyanodermella asteris; astin C; biofilms; immobilized-cell cultures; secondary metabolites.
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