Effect of madecassoside and catalpol in amelioration of insulin sensitivity in pancreatic (INS-1E) β-cell line

  • Nat Prod Res. 2021 Nov;35(22):4627-4631. doi: 10.1080/14786419.2019.1696794.
Safa Abdelgadir Mohamed Elhassan  1 ,  Mayuren Candasamy  2 ,  Tan Swee Ching  1 ,  Yap Kah Heng  1 ,  Subrat Kumar Bhattamisra  2
Affiliations
  • 1. School of Postgraduate Studies, International Medical University, Kuala Lumpur, Malaysia.
  • 2. Department of Life Sciences, School of Pharmacy, International Medical University, Kuala Lumpur, Malaysia.
PMID: 31797687 DOI: 10.1080/14786419.2019.1696794
Abstract

Currently, type 2 diabetes mellitus (T2D) has emerged as global burden disease. Herbal drugs with antidiabetic activities are attracting the attention. Madecassoside and catalpol are herbal compounds having strong antioxidant and glucose lowering activity. Madecassoside and catalpol were investigated for their effect on Insulin sensitivity using pancreatic INS-1E cells. Cytotoxicity of these compounds was evaluated by MTT assay. Glucose-stimulated Insulin secretion (GSIS) and expression of Insulin signalling proteins were studied in presence of madecassoside and catalpol. Results revealed that madecassoside and catalpol enhanced the GSIS without cytotoxic effect. Madecassoside (30 µM) and catalpol (40 µM) increased the Insulin secretion in response to high glucose (16.7 mM) stimulation. Subsequently, madecassoside and catalpol showed elevated expression of p-IRS-1, Akt, and p-Akt proteins. Madecassoside and catalpol after 24 h of incubation in pancreatic INS-1E cells with high glucose concentration (30 mM) ameliorated the Insulin secretion.

Keywords
Catalpol; Madecassoside; Pancreatic INS-1E cells.
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