Eckol from Ecklonia cava Suppresses Immunoglobulin E-mediated Mast Cell Activation and Passive Cutaneous Anaphylaxis in Mice

  • Nutrients. 2020 May 9;12(5):1361. doi: 10.3390/nu12051361.
Eui Jeong Han  1 ,  Hyun-Soo Kim  2 ,  K K A Sanjeewa  3 ,  K H I N M Herath  4 ,  You-Jin Jeon  3 ,  Youngheun Jee  4 ,  Jeongjun Lee  5 ,  Taehee Kim  5 ,  Sun-Yup Shim  6 ,  Ginnae Ahn  1  7
Affiliations
  • 1. Department of Food Technology and Nutrition, Chonnam National University, Yeosu 59626, Korea.
  • 2. National Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101 gil, Janghang-eup, Seocheon 33662, Korea.
  • 3. Department of Marine Life Science, Jeju National University, Jeju 63243, Korea.
  • 4. Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Korea.
  • 5. Naturetech, 29-8, Yongjeong-gil, Chopyeong-myeon, Jincheon-gun, Chungbuk 27858, Korea.
  • 6. Fisheries Science Institute, Chonnam National University, Daehak-Ro, Yeosu 59626, Korea.
  • 7. Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 59626, Korea.
PMID: 32397556 DOI: 10.3390/nu12051361
Abstract

Eckol, a precursor compound belonging to the dibenzo-1,4-dioxin class of phlorotannins, is a phloroglucinol derivative that exerts various activities. In the present study, we investigated the antiallergic effects of eckol isolated from the marine brown algae, Ecklonia cava using immunoglobulin E (IgE)/bovine serum albumin (BSA)-stimulated mouse bone marrow-derived cultured mast cells (BMCMC) and a mouse model of anaphylaxis. Eckol inhibited IgE/BSA-induced BMCMC degranulation by reducing β-hexosaminidase release. A flow cytometric analysis revealed that eckol decreases FcεRI expression on cell surface and IgE binding to the FcεRI in BMCMC. Moreover, eckol suppressed the production of the cytokines, interleukin (IL)-4, IL-5, IL-6, and IL-13 and the chemokine, thymus activation-regulated chemokine (TARC) by downregulating, IκB-α degradation and NF-κB nuclear translocation. Furthermore, it attenuated the passive cutaneous anaphylactic reaction induced by IgE/BSA-stimulation in the ear of BALB/c mice. These results suggest that eckol is a potential therapeutic candidate for the prevention and treatment of allergic disorders.

Keywords
Ecklonia cava; Eckol; bone marrow-derived cultured mast cells; immunoglobulin E; passive cutaneous anaphylaxis.
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