Synergistic stimulating effect of 2-hydroxymelatonin and BMP-4 on osteogenic differentiation in vitro

  • Biochem Biophys Res Commun. 2020 Jul 5;527(4):941-946. doi: 10.1016/j.bbrc.2020.04.126.
Sung Ho Lee  1 ,  Jin Wook Hwang  2 ,  Younho Han  3 ,  Kwang Youl Lee  4
Affiliations
  • 1. Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA.
  • 2. INSERM UMR-S 935, Université Paris Sud, 94800 Villejuif, France and ESTeam Paris Sud, Université Paris Sud, 94800, Villejuif, France.
  • 3. Department of Oral Pharmacology, College of Dentistry, Wonkwang University, Iksan, Republic of Korea. Electronic address: [email protected].
  • 4. College of Pharmacy & Research Institute of Drug Development, Chonnam National University, Gwangju, 500-757, Republic of Korea. Electronic address: [email protected].
PMID: 32439177 DOI: 10.1016/j.bbrc.2020.04.126
Abstract

2-Hydroxymelatonin is a metabolite produced when melatonin 2-hydroxylase catalyzes melatonin. Recent studies have reported the important roles of melatonin in bone metabolism. However, the roles of 2-hydroxymelatonin in bone metabolism remains poorly understood. The purpose of this study is to present evidence of the effect of 2-hydroxymelatonin on osteogenic differentiation in C2C12 cells. In this study, we demonstrated the synergistic stimulating effect of 2-hydroxymelatonin and bone morphogenetic protein (BMP)-4 on osteogenic differentiation in vitro, using Alkaline Phosphatase (ALP) staining, Alizarin red S (ARS) staining, qPCR, and luciferase reporter assay. The combination of 2-hydroxymelatonin and BMP-4 revealed a synergistic effect on osteogenic differentiation in vitro. This finding provides evidence that optimal concentrations of both 2-hydroxymelatonin and BMP-4 are beneficial for anabolic effects on bone in vitro.

Keywords
2-Hydroxymelatonin; BMP-4; Osteoblast differentiation.
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