D-Carvone inhibit cerebral ischemia/reperfusion induced inflammatory response TLR4/NLRP3 signaling pathway

  • Biomed Pharmacother. 2020 Dec:132:110870. doi: 10.1016/j.biopha.2020.110870.
Mengyuan Dai  1 ,  Lixiu Wu  1 ,  Kunqiang Yu  1 ,  Ri Xu  1 ,  Yanbin Wei  2 ,  Arunachalam Chinnathambi  3 ,  Tahani Awad Alahmadi  4 ,  Minya Zhou  5
Affiliations
  • 1. Department of Rehabilitation, The Second People's Hospital of Lishui, LiShui, ZheJiang, 323000, China.
  • 2. Thirteen Ward (Department of Respiratory & ICU), The Second People's Hospital of Lishui, LiShui, ZheJiang, 323000, China.
  • 3. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
  • 4. Department of Pediatrics, College of Medicine, King Saud University, Medical City, King Khalid University Hospital, PO Box-2925, Riyadh, 11461, Saudi Arabia.
  • 5. Department of Rehabilitation, The Second People's Hospital of Lishui, LiShui, ZheJiang, 323000, China. Electronic address: [email protected].
PMID: 33080468 DOI: 10.1016/j.biopha.2020.110870
Abstract

To explore the present treatment strategies for ischemic stroke lowered by ischemia-reperfusion (I/R) injury, to hypothesize the effect of d-Carvone on cerebral I/R brain injury induced neuroinflammation through oxidative stress markers mechanism via NRLP3 and TLR4 marker expressions in rat model. The rats were divided into four groups: Sham, I/R vehicle, I/R + D-carvone (10 mg/kg/bw), I/R + D-carvone (20 mg/kg/bw). Supplementation of d-carvone at dose of 10 and 20 m/kg/bw increased the water content, reduced infract volume, attenuated neurological score depicts, furthermore it had antioxidative, anti-inflammatory, and anti-apoptotic effects against cerebral I/R brain injury. In the brain tissues decreased proinflammatory cytokines IL-1β and TNF-α reduced interleukins IL-6, IL-4, IL-10 & VEGF dose dependently, and mRNA expressions of NLRP3, Caspase -1, TNF-α, ASC, IL-1β and TLR3 down regulated in cerebral I/R induced rats. Finally d- carvone can successfully improve the cerebral I/R induced rats neuroinflammation, in the hippocampus and cortical areas of the brain finally reduces cerebral I/R induced injury. These results were hypothesized that d-carvone contributed to cerebral stroke associated with the TLR3, giving an excellent therapeutic approach for cerebral I/R brain injury.

Keywords
Cerebral ischemia/reperfusion injury; D-Carvone; NLRP3; Neuroinflammation; Oxidative stress; TLR4.
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