Is the penetration of clindamycin into the masseter muscle really enough to treat odontogenic infections?

  • Clin Oral Investig. 2021 May;25(5):3257-3266. doi: 10.1007/s00784-020-03656-z.
Paula I Faggion  1 ,  Gabriela Isoton  2 ,  Eduarda Possa  2 ,  Leandro Tasso  3  4
Affiliations
  • 1. Laboratory of Pharmacokinetics, Health Sciences Postgraduate Program, University of Caxias do Sul, Caxias do Sul, Rio Grande do Sul, Brazil.
  • 2. College of Pharmacy, University of Caxias do Sul, Caxias do Sul, Rio Grande do Sul, Brazil.
  • 3. Laboratory of Pharmacokinetics, Health Sciences Postgraduate Program, University of Caxias do Sul, Caxias do Sul, Rio Grande do Sul, Brazil. [email protected].
  • 4. College of Pharmacy, University of Caxias do Sul, Caxias do Sul, Rio Grande do Sul, Brazil. [email protected].
PMID: 33128135 DOI: 10.1007/s00784-020-03656-z
Abstract

Objectives: This study aims to determine the penetration of clindamycin into the masseter muscle of rats by microdialysis and correlate with the main Microorganisms involved in odontogenic infections.

Materials and methods: Tissue concentrations of clindamycin in healthy muscle tissue were measured by microdialysis after administration of a single intravenous dose of 51 mg/kg and multiple doses of 17 mg/kg (8/8 h). It was quantified in plasma after a single administration of 51 mg/kg. Microdialysis samples were collected at 30-min intervals and clindamycin was assayed by LC-MS. Pharmacokinetic parameters and tissue penetration were determined. Pharmacokinetic/pharmacodynamic index (ƒ%T > minimum inhibitory concentration (MIC)) was considered to assess dosing regimens.

Results: The pharmacokinetic parameters determined by non-compartmental plasma analysis for the dose of 51 mg/kg were similar to that determined by compartmental analysis. The maximum free interstitial concentration (Cmax) of clindamycin in muscle tissue was 14.20 (10.63-14.89) and 4.82 (3.35-6.66) mg/L for 51 mg/kg and 17 mg/kg 8/8 h, respectively. In addition, the area under the curve (AUC0-inf) for plasma and tissue of clindamycin were 44.78 (28.82-65.65) and 16.54 (13.83-18.35) h.mg/L for 51 mg/kg, respectively, and the tissue penetration factor determined was 1.10. Considering that the main bacteria that cause odontogenic infections generally present MIC ≤ 0.5 mg/L, the ƒ%T > MIC index is reached when the dose regimen of 17 mg/kg 8/8 h is employed.

Conclusions: This investigation showed that clindamycin excellently penetrates muscle tissue of rats. It provides effective Antibacterial concentrations at the target site when 17 mg/kg 8/8 h is employed and can be applied to treat the main bacteria causing odontogenic infections.

Clinical relevance: It reinforces the use of clindamycin in odontogenic infections with significant tissue penetration.

Keywords
Clindamycin; Microdialysis; Odontogenic infections; Pharmacokinetics; Tissue penetration.
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