The ZAR1 resistosome is a calcium-permeable channel triggering plant immune signaling

  • Cell. 2021 Jun 24;184(13):3528-3541.e12. doi: 10.1016/j.cell.2021.05.003.
Guozhi Bi  1 ,  Min Su  2 ,  Nan Li  2 ,  Yu Liang  3 ,  Song Dang  2 ,  Jiachao Xu  2 ,  Meijuan Hu  1 ,  Jizong Wang  4 ,  Minxia Zou  5 ,  Yanan Deng  2 ,  Qiyu Li  2 ,  Shijia Huang  6 ,  Jiejie Li  5 ,  Jijie Chai  7 ,  Kangmin He  8 ,  Yu-Hang Chen  9 ,  Jian-Min Zhou  10
Affiliations
  • 1. State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
  • 2. State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
  • 3. State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
  • 4. State Key Laboratory of Protein and Plant Gene Research, School of Advanced Agricultural Sciences, Peking University, Beijing 100871, China.
  • 5. Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Science, Beijing Normal University, Beijing 100875, China; Key Laboratory of Cell Proliferation and Regulation of Ministry of Education, College of Life Science, Beijing Normal University, Beijing 100875, China.
  • 6. Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • 7. Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China; Max-Planck Institute for Plant Breeding Research, Cologne, Germany; Institute of Biochemistry, University of Cologne, Zuelpicher Str. 47, 50674 Cologne, Germany. Electronic address: [email protected].
  • 8. State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: [email protected].
  • 9. State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: [email protected].
  • 10. State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, China; CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: [email protected].
PMID: 33984278 DOI: 10.1016/j.cell.2021.05.003
Abstract

Nucleotide-binding, leucine-rich repeat receptors (NLRs) are major immune receptors in Plants and Animals. Upon activation, the Arabidopsis NLR protein ZAR1 forms a pentameric resistosome in vitro and triggers immune responses and cell death in Plants. In this study, we employed single-molecule imaging to show that the activated ZAR1 protein can form pentameric complexes in the plasma membrane. The ZAR1 resistosome displayed ion channel activity in Xenopus oocytes in a manner dependent on a conserved acidic residue Glu11 situated in the channel pore. Pre-assembled ZAR1 resistosome was readily incorporated into planar lipid-bilayers and displayed calcium-permeable cation-selective channel activity. Furthermore, we show that activation of ZAR1 in the plant cell led to Glu11-dependent CA2+ influx, perturbation of subcellular structures, production of Reactive Oxygen Species, and cell death. The results thus support that the ZAR1 resistosome acts as a calcium-permeable cation channel to trigger immunity and cell death.

Keywords
Arabidopsis; NLR; Pseudomonas; Xanthomonas; calcium channel; cell death; disease resistance; plant immunity; resistosome; single-molecule imaging.
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