Colorimetric and fluorescent turn-on detection of chloride ions with ionophore and BODIPY: Evaluation with nanospheres and cellulose paper

Here, we present the optical detection of chloride ions with co-extraction based anion-selective optodes containing a BODIPY derivative (BDP-OH) and the ionophore In(OEP)Cl. Spectroscopic studies suggested that BDP-OH and In(OEP)Cl formed an adduct BDP-O-In(OEP), which was converted to BDP-OH and In(OEP)Cl upon increasing sample Cl^- concentration, and induced signal changes in both fluorescence and absorbance modes. The method was evaluated in polystyrene-graft-poly(ethylene oxide) (PS-PEO) nanospheres (CA. 40 nm in diameter) and on cellulose paper. In contrast to Cl^- probes based on fluorescence quenching, the nanospheres exhibited turn-on fluorescence and ratiometric absorbance responses to a tunable Cl^- concentration range (10 μM-1 M). Through fluorescence microscopy, the nanospheres were able to respond to Cl^- concentration changes in HeLa cells. Cellulose paper-based Cl^- optodes with colorimetric response were successfully used to measure Cl^- in artificial sweat, providing a potential analytical tool for clinical diagnosis of cystic fibrosis.