ε-Viniferin and α-viniferin alone or in combination induced apoptosis and necrosis in osteosarcoma and non-small cell lung cancer cells

  • Food Chem Toxicol. 2021 Dec:158:112617. doi: 10.1016/j.fct.2021.112617.
Cheng Huang  1 ,  Zi-Jun Lin  2 ,  Cheng-Ju Lee  2 ,  Wei-Han Lai  3 ,  Jui-Chieh Chen  4 ,  Hsiu-Chen Huang  5
Affiliations
  • 1. Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan.
  • 2. Center for Teacher Education, National Tsing Hua University, Hsinchu, Taiwan; Department of Applied Science, National Tsing Hua University, Nanda Campus, Hsinchu, Taiwan.
  • 3. Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming Chiao Tung University, Taipei, 11221, Taiwan; Center for Teacher Education, National Tsing Hua University, Hsinchu, Taiwan; Department of Applied Science, National Tsing Hua University, Nanda Campus, Hsinchu, Taiwan.
  • 4. Department of Biochemical Science and Technology, National Chiayi University, Chiayi City, 60004, Taiwan. Electronic address: [email protected].
  • 5. Center for Teacher Education, National Tsing Hua University, Hsinchu, Taiwan; Department of Applied Science, National Tsing Hua University, Nanda Campus, Hsinchu, Taiwan. Electronic address: [email protected].
PMID: 34728247 DOI: 10.1016/j.fct.2021.112617
Abstract

This study investigated the effects and molecular mechanisms of ε-viniferin and α-viniferin in non-small cell lung Cancer cell line A549, melanoma cell line A2058, and osteosarcoma cell lines HOS and U2OS. Results showed ε-viniferin having antiproliferative effects on HOS, U2OS, and A549 cells. Compared with ε-viniferin at the same concentration, α-viniferin had higher antiproliferative effects on HOS cells, but not the same effect on U2OS and A549 cells. Lower dose combination of α-viniferin and ε-viniferin had more synergistic effects on A549 cells than either drug alone. α-Viniferin induced Apoptosis in HOS cells by decreasing expression of phospho-c-Jun-N-terminal kinase 1/2 (p-JNK1/2) and increasing expression of cleaved Poly (ADP-ribose) polymerase (PARP), whereas α-viniferin in combination with ε-viniferin induced Apoptosis in A549 cells by decreasing expression of phospho-protein kinase B (p-AKT) and increasing expression of cleaved PARP and cleaved Caspase-3. ε-Viniferin and α-viniferin have not been studied using in vivo tumor models for Cancer. This research is the first showing that ε-viniferin treatment resulted in significant inhibition of tumor growth in A549-cell xenograft-bearing nude mice compared with the control group. Consequently, ε-viniferin and α-viniferin may prove to be new approaches and effective therapeutic agents for osteosarcoma and lung Cancer treatment.

Keywords
Apoptosis; Lung cancer; Nude mice; Osteosarcoma; Viniferin.
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