ε-Viniferin
Based on 1 publication(s) in Google Scholar
ε-Viniferin (epsilon-Viniferin), the dimer of Resveratrol and can be isolated from Vitis vinifera, displays a potent inhibitory for all the CYP activities, with Ki values from 0.5-20 μM. ε-Viniferin possesses potent antioxidant, anti-inflammatory, anti-diabetic, and anti-neurodegenerative capacity.
For research use only. We do not sell to patients.
- Purity: 99.15%
- CAS No.: 62218-08-0
- Formula: C28H22O6
- Molecular Weight:454.47
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) ε-Viniferin
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Biological Activity
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CYP2B6 3 μM (Ki, (BROD)) |
CYP1A2 5 μM (Ki, (EROD)) |
CYP3A4 10 μM (Ki, (TST)) |
CYP4A 15 μM (IC50, (LwOH)) |
CYP2E1 25 μM (IC50, (CHZ)) |
CYP2A6 60 μM (Ki, (COH)) |
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Cell Line
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Type | Value | Description | References |
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| A549 | CC50 |
54.7 μM
Compound: (+)-Epsilon-Viniferin
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Cytotoxicity against human A549 cells assessed as reduction in cell viability by Alamar blue assay
Cytotoxicity against human A549 cells assessed as reduction in cell viability by Alamar blue assay
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[PMID: 32652408] |
| BTI-TN-5B1-4 | IC50 |
19.6 μM
Compound: Epsilon-Viniferin
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Inhibition of human recombinant MAO-A expressed in baculovirus infected BTI-TN-5B1-4 insect cells using p-tyramine as substrate after 15 mins by resorufin dye-based spectrometric analysis
Inhibition of human recombinant MAO-A expressed in baculovirus infected BTI-TN-5B1-4 insect cells using p-tyramine as substrate after 15 mins by resorufin dye-based spectrometric analysis
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[PMID: 30686752] |
| Huh-7.5 | CC50 |
>10 μM
Compound: (+)-Epsilon-Viniferin
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Cytotoxicity against HCV RNA-transfected human Huh7.5 cells assessed as reduction in cell viability
Cytotoxicity against HCV RNA-transfected human Huh7.5 cells assessed as reduction in cell viability
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[PMID: 32652408] |
| Huh-7.5 | CC50 |
>10 μM
Compound: (+)-Epsilon-Viniferin
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Cytotoxicity against human Huh7.5 cells infected with Hepatitis C virus assessed as reduction in cell viability
Cytotoxicity against human Huh7.5 cells infected with Hepatitis C virus assessed as reduction in cell viability
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[PMID: 32652408] |
ε-Viniferin (1-15 μM, 24-72 h) inhibits proliferation of HOS, U2OS, and A549 cells[3].
ε-Viniferin (20 μM, 24 h) induces DNA fragmentation and nuclear condensation in HOS, U2OS, A549 cells[3].
ε-Viniferin (10 μM, 1 h) inhibits TGF-β1 induced EMT, invasion and migration, and reverses TGF-β1-induced ROS, MMP2, vimentin, Zeb1, Snail, p-SMAD2, p-SMAD3, and ABCG2 expression in A549 cells[4].
ε-Viniferin (10 μM, 3 min) inhibits fMLP (0.1 μM)-induced superoxide anion production in human neutrophils, as well as fMLP-induced phosphorylation of ERK, Akt, Src or intracellular calcium mobilization[5].
ε-Viniferin (50 nM-10 μM, 48 h) protects cells against Rotenone (HY-B1756)-induced neurotoxicity in SH-SY5Y cells via the SIRT3/FOXO3 pathway[6].
ε-Viniferin (1 μM, 24 h) inhibits Rotenone (HY-B1756)-induced mitochondrial depolarization and oxidative stress in SH-SY5Y cells[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549 cells
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Concentration:10 μM
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Incubation Time:1 h
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Result:Inhibited TGF-β1-induced MMP2, vimentin, Zeb1, p-SMAD2, p-SMAD3, and ABCG2 expression.
ε-Viniferin (0.2% in diet, 4 weeks) prevents diet-induced obesity in mice[7].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:A549 cell xenograft nude mice model[3]
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Dosage:5 mg/kg
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Administration:i.p., 5 days per week for 4 weeks
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Result:Inhibited tumor weight and volume.
Induced apoptosis in tumor sections.
Decreased serum ALT, AST, bilirubin, and creatinine levels.
Chemical Information
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CAS No. 62218-08-0
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Appearance Solid
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Molecular Weight 454.47
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Formula C28H22O6
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Color Off-white to gray
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SMILES
OC1=CC([C@H]2[C@H](C3=CC=C(O)C=C3)OC4=CC(O)=CC(/C=C/C5=CC=C(O)C=C5)=C24)=CC(O)=C1
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Synonyms
epsilon-Viniferin
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Arthritis Res Ther
Sirt3 improves monosodium urate crystal-induced inflammation by suppressing Acod1 expression. [Abstract]2023 Jul 19;25(1):121. PMID: 37468929
Solvent & Solubility
DMSO : ≥ 50 mg/mL (110.02 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.75 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (2.75 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (287 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Piver B, et al. Differential inhibition of human cytochrome P450 enzymes by epsilon-viniferin, the dimer of resveratrol: comparison with resveratrol and polyphenols from alcoholized beverages. Life Sci. 2003 Jul 18;73(9):1199-213. [Content Brief]
[3]. Huang C, et al. ε-Viniferin and α-viniferin alone or in combination induced apoptosis and necrosis in osteosarcoma and non-small cell lung cancer cells. Food Chem Toxicol. 2021 Dec;158:112617. [Content Brief]
[4]. Chiou WC, et al. α-Viniferin and ε-Viniferin Inhibited TGF-β1-Induced Epithelial-Mesenchymal Transition, Migration and Invasion in Lung Cancer Cells through Downregulation of Vimentin Expression. Nutrients. 2022 May 30;14(11):2294. [Content Brief]
[5]. Liao HR, et al. The anti-inflammatory effect of ε-viniferin by specifically targeting formyl peptide receptor 1 on human neutrophils. Chem Biol Interact. 2021 Aug 25;345:109490. [Content Brief]
[6]. Zhang S, et al. Neuroprotective mechanisms of ε-viniferin in a rotenone-induced cell model of Parkinson's disease: significance of SIRT3-mediated FOXO3 deacetylation. Neural Regen Res. 2020 Nov;15(11):2143-2153. [Content Brief]
[7]. Ohara K, et al. ε-Viniferin, a resveratrol dimer, prevents diet-induced obesity in mice. Biochem Biophys Res Commun. 2015 Dec 25;468(4):877-82. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2004 mL | 11.0018 mL | 22.0037 mL | 55.0091 mL |
| 5 mM | 0.4401 mL | 2.2004 mL | 4.4007 mL | 11.0018 mL | |
| 10 mM | 0.2200 mL | 1.1002 mL | 2.2004 mL | 5.5009 mL | |
| 15 mM | 0.1467 mL | 0.7335 mL | 1.4669 mL | 3.6673 mL | |
| 20 mM | 0.1100 mL | 0.5501 mL | 1.1002 mL | 2.7505 mL | |
| 25 mM | 0.0880 mL | 0.4401 mL | 0.8801 mL | 2.2004 mL | |
| 30 mM | 0.0733 mL | 0.3667 mL | 0.7335 mL | 1.8336 mL | |
| 40 mM | 0.0550 mL | 0.2750 mL | 0.5501 mL | 1.3752 mL | |
| 50 mM | 0.0440 mL | 0.2200 mL | 0.4401 mL | 1.1002 mL | |
| 60 mM | 0.0367 mL | 0.1834 mL | 0.3667 mL | 0.9168 mL | |
| 80 mM | 0.0275 mL | 0.1375 mL | 0.2750 mL | 0.6876 mL | |
| 100 mM | 0.0220 mL | 0.1100 mL | 0.2200 mL | 0.5501 mL |
ε-Viniferin Related Classifications
HY-N3841 Related Classifications
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