A responsive fluorescent probe for detecting and imaging pyruvate kinase M2 in live cells

  • Chem Commun (Camb). 2022 Jun 1;58(45):6494-6497. doi: 10.1039/d2cc01211a.
Ya Zhang  1 ,  Huishan Li  1 ,  Hailing Mai  1 ,  Dong Luo  1 ,  Xinpei Ji  1 ,  Zhengting Liu  1 ,  Shiyong Peng  1 ,  Xuetao Xu  1 ,  Yinghui Zhang  1  2 ,  Rongfeng Lan  3 ,  Hongguang Li  1  2
Affiliations
  • 1. Guangdong Provincial Key Laboratory of, Large Animal Models for Biomedicine, School of Biotechnology and Health Science, Wuyi University, Jiangmen 529020, China. [email protected].
  • 2. South China Institute of Large Animal Models for Biomedicine, Wuyi University, Jiangmen 529020, China.
  • 3. College of Basic medical sciences, Shenzhen University Health Science Center, Shenzhen 518060, China. [email protected].
PMID: 35574810 DOI: 10.1039/d2cc01211a
Abstract

In this study, we designed and tested fluorescent probe zy-2 for specific and responsive imaging of Pyruvate Kinase M2 (PKM2), which can be excited by 419 nm light. A 17-fold enhancement in the responsive emission upon zy-2's binding to PKM2 was observed, imaging of which was successfully recorded in a time- and concentration-dependent manner in PKM2-positive cells. Thus, we obtained a responsive fluorescent probe for the specific and sensitive detection of PKM2, which is innovative in design and applicable to the detection of Cancer cells.

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