Antiaging effect of inotodiol on oxidative stress in human dermal fibroblasts

  • Biomed Pharmacother. 2022 Sep:153:113311. doi: 10.1016/j.biopha.2022.113311.
Seung Hoon Lee  1 ,  Gun-Woo Won  2 ,  Seung-Hyeon Choi  1 ,  Mi-Yoon Kim  3 ,  Cheong-Hae Oh  3 ,  Jong-Tae Park  4 ,  Jong-Il Park  5
Affiliations
  • 1. Department of Biochemistry, College of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea; Translational Immunology Institute, Chungnam National University College of Medicine, Daejeon, Republic of Korea.
  • 2. Department of Biochemistry, College of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea; Brain Korea 21 FOUR Project for Medical Science, Chungnam National University, Daejeon, Republic of Korea.
  • 3. Department of Biochemistry, College of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea.
  • 4. Department of Food Science and Technology, Chungnam National University, Daejeon 34134, Republic of Korea; CARBOEXPERT Inc., Daejeon 34134, Republic of Korea. Electronic address: [email protected].
  • 5. Department of Biochemistry, College of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea; Translational Immunology Institute, Chungnam National University College of Medicine, Daejeon, Republic of Korea; Brain Korea 21 FOUR Project for Medical Science, Chungnam National University, Daejeon, Republic of Korea. Electronic address: [email protected].
PMID: 35759867 DOI: 10.1016/j.biopha.2022.113311
Abstract

Oxidative damage is one of the major causes of human skin aging. Inotodiol is a lanostane triterpenoid that demonstrates Antiviral, Anticancer, and anti-inflammatory activities. Previous studies have reported that inotodiol also has antiallergic effects. However, whether inotodiol inhibits oxidative stress-induced human skin aging is not known. Stimulation of human dermal fibroblast cells with hydrogen peroxide is related to skin aging. Inotodiol inhibited the expression of mitogen-activated protein kinase (MAPK) and NADPH Oxidase 5 (NOX5). Moreover, inotodiol effectively decreased nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), as well as nitric oxide (NO), Reactive Oxygen Species (ROS), cyclooxygenase-2 (COX-2), and cytokines such as IL-1β, IL-6, and TNF-α. Based on our results, inotodiol protects human dermal fibroblast by preventing MAPK-NOX5 and NF-κB activation and attenuates the expression of aging genes. Inotodiol may therefore be considered a potential candidate for developing natural antiaging products, because it protects the human skin from oxidative stress-induced skin aging by inhibiting the MAPK-NOX5 and NF-κB signaling pathways.

Keywords
Human dermal fibroblats; Inotodiol; MAPK; NADPH Oxidase 5; Skin aging.
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