One-pot synthesis of α-Linolenic acid nanoemulsion-templated drug-loaded silica mesocomposites as efficient bactericide against drug-resistant Mycobacterium tuberculosis

  • Eur J Pharm Sci. 2022 Sep 1;176:106261. doi: 10.1016/j.ejps.2022.106261.
Ping Zhu  1 ,  Ling Cai  1 ,  Qiao Liu  2 ,  Shanwu Feng  3 ,  Hongjie Ruan  3 ,  Li Zhang  1 ,  Liuzhu Zhou  1 ,  Huijun Jiang  4 ,  Hongsheng Wang  5 ,  Jianming Wang  6 ,  Jin Chen  7
Affiliations
  • 1. Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
  • 2. Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China; Department of Chronic Communicable Disease, Center for Disease Control and Prevention of Jiangsu Province, Nanjing, China.
  • 3. Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, 123 Tianfei Lane, Nanjing 210004, China.
  • 4. School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
  • 5. Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China; Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, St. 12 Jiangwangmiao, Nanjing 210042, China. Electronic address: [email protected].
  • 6. Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China. Electronic address: [email protected].
  • 7. Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China; Jiangsu Province Engineering Research Center of Antibody Drug, Key Laboratory of Antibody Technique of National Health Commission, Nanjing Medical University, Nanjing 211166, China; The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China; Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Tarim University, Alar 843300, China. Electronic address: [email protected].
PMID: 35840102 DOI: 10.1016/j.ejps.2022.106261
Abstract

Nowadays, pathogenic Infection has posed a severe threat to the public health and environmental sanitation, urging a continuous search of efficacious and safe bactericidal agents of various formulated forms. Here, a facile one-pot hydrothermal preparation of mesoporous silica nanoparticles using ultrasonication-assisted nanoemulsion of α-Linolenic acid (α-LA) as template was developed. The formed silica mesocomposite at water/fatty-acid surface provides an easy yet green synthesis route, which can be generalized for the further encapsulation of hydrophobic drugs such as antimycobacterial Rifampicin (RIF). The obtained α-LA nanoemulsion-templated silica nanoparticles (LNS NPs), with a weight content of ∼17% α-LA in the composite, showed apparent Antibacterial effect against Staphylococcus aureus (S. aureus). By comparison, the removal of α-LA from the silica nanoparticles (LNS-1 NPs) resulted in the composite of enlarged pore size with negligible bactericidal activities. Notably, the Isoniazide (INH) and Rifampicin (RIF)-encapsulated LNS NPs exhibited outstanding antimycobacterial activity against both drug-sensitive and drug-resistant Mycobacterium tuberculosis (M. tuberculosis). The obtained highly biocompatible, biosafe and low-energy consumptive α-LA-contained mesostructured silica-based bactericide holds promising therapeutic potentials to tackle the emerging drug-resistant infectious microbes.

Keywords
Antibacterial agent; Drug-resistant Mycobacterium tuberculosis; Fatty acid; Mesoporous silica nanoparticles; Nanoemulsions.
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