Generation of expandable cardiovascular progenitor cells from mouse and human fibroblasts via direct chemical reprogramming
- STAR Protoc. 2022 Dec 16;3(4):101908. doi: 10.1016/j.xpro.2022.101908.
Affiliations
- 1. School of Health and Life Sciences, University of Health and Rehabilitation Sciences, Qingdao City, Shandong Province 266071, China. Electronic address: [email protected].
- 2. Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, China; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou 510080, China.
- 3. Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, China; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou 510080, China. Electronic address: [email protected].
PMID: 36595887
DOI: 10.1016/j.xpro.2022.101908
Abstract
Here, we present a protocol to reprogram mouse and human fibroblasts into expandable cardiovascular progenitor cells (CPCs) via a defined small-molecule treatment. We describe steps to prepare fibroblasts and generate the chemically induced CPCs (ciCPCs), followed by expansion and differentiation of the ciCPCs. These cells can self-renew in the long term, faithfully retaining the CPC phenotype and cardiovascular differentiation capacity. This protocol provides an autologous and expandable cardiovascular cell source, which may find uses in Cardiovascular Disease modelling, drug discovery, and cardiac cell therapy. For complete details on the use and execution of this protocol, please refer to Wang et al. (2022).1.
Keywords
Cell Biology; Cell Differentiation; Cell culture; Cell-based Assays; Stem Cells.