Anti-inflammatory effects of NaB and NaPc in Acinetobacter baumannii-stimulated THP-1 cells via TLR-2/NF-κB/ROS/NLRP3 pathway

  • Acta Pharm. 2022 Oct 18;72(4):615-628. doi: 10.2478/acph-2022-0036.
Chen Shu  1  2 ,  Zhang Yan-Yan  3 ,  Zhang Hai  2 ,  Ding Long-Kun  4 ,  Xi Yue  4 ,  Yan Man  4 ,  Sun Chang  4 ,  Wu Liang  4 ,  Hu Hao  1
Affiliations
  • 1. General Surgery Department, The First Affiliated Hospital of Soochow University Suzhou, Jiangsu, 215006, China.
  • 2. General Surgery Department, Affiliated Hospital of Jiangsu University, Jiefang Road No. 438, Zhenjiang, Jiangsu 212000, China.
  • 3. Testing Center, Yangzhou University Wenhui Road No. 48, Yangzhou, Jiangsu 225009, China.
  • 4. School of Medicine, Jiangsu University Xuefu Road No. 301, Zhenjiang, Jiangsu 212013, China.
PMID: 36651366 DOI: 10.2478/acph-2022-0036
Abstract

This study evaluated the anti-inflammation effect of the three main short-chain fatty acids (SCFAs) on Acinetobacter baumannii-induced THP-1 cells. The three main SCFAs could inhibit A. baumannii-stimulated THP-1 cell NF-κB pathway activity and the expressions of NLRP3 inflamma-some and GSDMD, and increase Autophagy. The three main SCFAs, especially the sodium butyrate (NaB), had the effect of down-regulation of ROS and TLR-2 expression in THP-1 cells. NaB and sodium propionate (NaPc), but not sodium acetate (NaAc), dramatically suppressed IL-1β and IFN-γ expression. The results indicated that NaB and NaPc could significantly inhibit the inflammation of THP-1 cells induced by A. baumannii, and the inhibitory effect was in the order of NaB > NaPc > NaAC. NaB and NaPc may inhibit inflammation through TLR-2/NF-κB/ROS/NLRP3 signaling pathway.

Keywords
NLRP3; autophagy; inflammation; nuclear factor-κB (NF-κB); short-chain fatty acids (SCFAs).
Products