Detection of anti-feline infectious peritonitis virus activity of a Chinese herb extract using geneLEAD VIII, a fully automated nucleic acid extraction/quantitative PCR testing system
- J Vet Med Sci. 2023 Mar 30;85(4):443-446. doi: 10.1292/jvms.22-0185.
- 1. Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
- 2. Laboratory of Veterinary Infectious Diseases, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.
- 3. Iskra Industry Co., Ltd., Tokyo, Japan.
- 4. Department of Veterinary Hygiene, Veterinary School, Nippon Veterinary and Life Science University, Tokyo, Japan.
- 5. Precision System Science Co., Ltd., Chiba, Japan.
The geneLEAD VIII is a fully-automated nucleic acid extraction/quantitative PCR equipment developed by Precision System Science Co., Ltd., (PSS). To take advantage of its capability, we developed a quantitative assay system to measure growth of animal viruses. The system was used to assay one of the Chinese herbal extracts whose anti-malarial activities were previously reported and demonstrated its dose-dependent anti-viral activity against feline infectious peritonitis virus (FIPV), a feline coronavirus causing the fatal diseases in cats, and relatively low cell toxicity. The assay developed in this study is useful to screen Antiviral drugs and the anti-FIPV activity of the herbal extract identified have a potential to lead to development of new drugs against FIPV and Other coronaviruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
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Cat. No.Product NameDescriptionTargetResearch Area
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Research Areas: Inflammation/Immunology