Single-cell RNA sequencing highlights intratumor heterogeneity and intercellular network featured in adamantinomatous craniopharyngioma

  • Sci Adv. 2023 Apr 14;9(15):eadc8933. doi: 10.1126/sciadv.adc8933.
Yu Jiang  1 ,  Jinlong Yang  1 ,  Ruichao Liang  1 ,  Xin Zan  1 ,  Rangrang Fan  1 ,  Baoyin Shan  1 ,  Hao Liu  1 ,  Li Li  2 ,  Yue Wang  3 ,  Min Wu  4 ,  Xin Qi  1 ,  Hongxu Chen  1 ,  Qingqing Ren  1 ,  Zhiyong Liu  1 ,  Yuelong Wang  1 ,  Jing Zhang  1 ,  Peizhi Zhou  1 ,  Qiang Li  1 ,  Meng Tian  1 ,  Jinhao Yang  1 ,  Chaoyang Wang  1 ,  Xueying Li  1 ,  Shu Jiang  1 ,  Liangxue Zhou  1 ,  Gao Zhang  5 ,  Yaohui Chen  6 ,  Jianguo Xu  1
Affiliations
  • 1. Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, 610041, China.
  • 2. Institute of Clinical Pathology, West China Hospital, Sichuan University, Chengdu, 610041, China.
  • 3. Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan, 250000, China.
  • 4. Huaxi MR Research Center (HMRRC), Department of Radiology, Functional and Molecular Imaging Key Laboratory of Sichuan Province, West China Hospital, Sichuan University, Chengdu, 610041, China.
  • 5. Faculty of Dentistry, The University of Hong Kong, Sai Ying Pun, 999077, Hong Kong.
  • 6. Department of Thoracic Surgery/Institute of Thoracic Oncology, West China Hospital, Sichuan University, Chengdu, 610041, China.
PMID: 37043580 DOI: 10.1126/sciadv.adc8933
Abstract

Despite improvements in microscopically neurosurgical techniques made in recent years, the prognosis of adamantinomatous craniopharyngioma (ACP) is still unsatisfactory. Little is known about cellular atlas and biological features of ACP. Here, we carried out integrative analysis of 44,038 single-cell transcriptome profiles to characterize the landscape of intratumoral heterogeneity and tumor microenvironment (TME) in ACP. Four major neoplastic cell states with distinctive expression signatures were defined, which further revealed the histopathological features and elucidated unknown cellular atlas of ACP. Pseudotime analyses suggested potential evolutionary trajectories between specific neoplastic cell states. Notably, a distinct oligodendrocyte lineage was identified in ACP, which was associated with immunological infiltration and neural damage. In addition, we described a tumor-centric regulatory network based on intercellular communication in TME. Together, our findings represent a unique resource for deciphering tumor heterogeneity of ACP, which will improve clinical diagnosis and treatment strategies.

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