Phenazine methylsulfate
Based on 1 publication(s) in Google Scholar
Phenazine methylsulfate is a free radical generator that can act as an electron transfer reactant in cell viability assays. It also has insecticidal properties. Furthermore, Phenazine methylsulfate induces oxidative DNA damage and cell apoptosis, showing antitumor activity.
For research use only. We do not sell to patients.
- Purity: 98.71%
- CAS No.: 299-11-6
- Formula: C14H14N2O4S
- Molecular Weight:306.34
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Phenazine methylsulfate
MoreAll Antibiotic Isoforms
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Biological Activity
Phenazine methyl sulfate stimulates proton transport by promoting photosynthetic electron flow[1].
Phenazine methyl sulfate (10 μM, 2-24 h) can quickly disrupt and clear Leishmania amastigotes from macrophages without causing significant harm to the host cells[2].
Phenazine methyl sulfate (2.5-20 μM, 24 h) can inhibit or kill Leishmania promastigotes in a dose-dependent manner[2].
Phenazine methyl sulfate (0-10 μM, 24 h) induces apoptosis in A375, G361, and LOX cells[3].
Phenazine methyl sulfate (5-10 μM, 0-24 h) leads to the upregulation of heat shock, oxidative, and genotoxic stress response gene expression in A375 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Macrophages infected with Leishmania m. amazonensis amastigotes
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Concentration:10 μM
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Incubation Time:2, 6, 24 h
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Result:Reduced parasite vesicles.
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Cell Line:A375, G361, LOX
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Concentration:0, 1, 5, 10 μM
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Incubation Time:24 h
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Result:Induced cell death.
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Cell Line:A375
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Concentration:5, 10 μM
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Incubation Time:0, 1, 3, 6, 12, 18, 24 h
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Result:Up-regulated the expression of Hsp70, antioxidant response regulatory transcription factors NRF2 and HO-1, up-regulated Bax, PUMA, cleaved PARP1, EGR1, and down-regulated Mcl-1, Bcl-2.
Chemical Information
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CAS No. 299-11-6
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Appearance Solid
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Molecular Weight 306.34
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Formula C14H14N2O4S
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Color Yellow to brown
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SMILES
C[N+]1=C2C=CC=CC2=NC3=C1C=CC=C3.O=S(OC)([O-])=O
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Synonyms
5-Methylphenazinium methylsulfate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Sci Adv
Single-cell RNA sequencing highlights intratumor heterogeneity and intercellular network featured in adamantinomatous craniopharyngioma. [Abstract]2023 Apr 14;9(15):eadc8933. PMID: 37043580
Solvent & Solubility
H2O : 100 mg/mL (326.43 mM; Need ultrasonic)
DMSO : 100 mg/mL (326.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.79 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (6.79 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.08 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (326.43 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. G E Edwards, et al. Action of phenazine methyl sulfate, inhibitors, and uncouplers on the light-induced proton transport by cells ofRhodospirillum rubrum. J Membr Biol. 1970 Dec;2(1):95-107. [Content Brief]
[2]. Anh B Hua, et al. Repurposing the Electron Transfer Reactant Phenazine Methosulfate (PMS) for the Apoptotic Elimination of Malignant Melanoma Cells through Induction of Lethal Oxidative and Mitochondriotoxic Stress. Cancers (Basel). 2019 Apr 27;11(5):590. [Content Brief]
[3]. E Pontiki, et al. Evaluation of anti-inflammatory and antioxidant activities of copper (II) Schiff mono-base and copper(II) Schiff base coordination compounds of dien with heterocyclic aldehydes and 2-amino-5-methyl-thiazole. J Enzyme Inhib Med Chem. 2008 Dec;23(6):1011-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.2643 mL | 16.3216 mL | 32.6431 mL | 81.6079 mL |
| 5 mM | 0.6529 mL | 3.2643 mL | 6.5286 mL | 16.3216 mL | |
| 10 mM | 0.3264 mL | 1.6322 mL | 3.2643 mL | 8.1608 mL | |
| 15 mM | 0.2176 mL | 1.0881 mL | 2.1762 mL | 5.4405 mL | |
| 20 mM | 0.1632 mL | 0.8161 mL | 1.6322 mL | 4.0804 mL | |
| 25 mM | 0.1306 mL | 0.6529 mL | 1.3057 mL | 3.2643 mL | |
| 30 mM | 0.1088 mL | 0.5441 mL | 1.0881 mL | 2.7203 mL | |
| 40 mM | 0.0816 mL | 0.4080 mL | 0.8161 mL | 2.0402 mL | |
| 50 mM | 0.0653 mL | 0.3264 mL | 0.6529 mL | 1.6322 mL | |
| 60 mM | 0.0544 mL | 0.2720 mL | 0.5441 mL | 1.3601 mL | |
| 80 mM | 0.0408 mL | 0.2040 mL | 0.4080 mL | 1.0201 mL | |
| 100 mM | 0.0326 mL | 0.1632 mL | 0.3264 mL | 0.8161 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Phenazine methylsulfate Related Classifications
HY-W004520 Related Classifications
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