Fangchinoline Exerts Anticancer Effects on Colorectal Cancer Cells by Evoking Cell Apoptosis via Endoplasmic Reticulum Stress

  • Bull Exp Biol Med. 2023 Apr 13;1-8. doi: 10.1007/s10517-023-05761-x.
Xiuling Jiang  1 ,  Yougen Hu  2 ,  Jianming Peng  1 ,  Xue Luo  1 ,  Landi Su  1 ,  Yuanjie Tang  3
Affiliations
  • 1. Medical School of Yangzhou Polytechnic College, Yangzhou, China.
  • 2. Department of Surgery of Jiangsu, Provincial Corps Hospital, Yangzhou, China.
  • 3. Department of Surgery of Jiangsu, Provincial Corps Hospital, Yangzhou, China. [email protected].
PMID: 37052856 DOI: 10.1007/s10517-023-05761-x
Abstract

We studied the anti-tumor effect of fangchinoline (FAN) against human colorectal Cancer cell lines CCL-244 and SW480 and analyzed the mechanism of FAN action. The cell viability and Apoptosis were assessed by MTT test and Annexin V-PI staining; Caspase-3 activity was measured by Western blotting. The expression of endoplasmic reticulum stress-related proteins was assessed by Real-Time PCR, Western blotting, and gene transfection. It was found that FAN inhibited cell growth and induced Apoptosis in human colorectal Cancer cell lines CCL-244 and SW480 in a dose-dependent manner. The Caspase-3 inhibitor Ac-DEVD-CHO could reverse the inhibitory effect of FAN. Moreover, FAN significantly increased the expression of endoplasmic reticulum stress-related proteins p-PERK, p-eIF2α, ATF4, and CHOP in CCL-244 and SW480 cells. In addition, endoplasmic reticulum stress inhibitor 4-phenylbutyric acid or CHOP knockdown could prevent FAN-induced Apoptosis. Thus, FAN induced Apoptosis of human colorectal Cancer through activation of endoplasmic reticulum stress.

Keywords
CHOP gene; apoptosis; colorectal cancer; endoplasmic reticulum stress; fangchinoline.
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