PLGA-based nanocarriers for combined delivery of colchicine and purpurin 18 in cancer therapy: Multimodal approach employing cancer cell spheroids

  • Int J Pharm. 2024 Apr 26:657:124170. doi: 10.1016/j.ijpharm.2024.124170.
Alma Lucia Villela Zumaya  1 ,  Vladimíra Svobodová Pavlíčková  2 ,  Silvie Rimpelová  3 ,  Markéta Štějdířová  1 ,  Michal Fulem  1 ,  Ivana Křížová  4 ,  Pavel Ulbrich  2 ,  Pavel Řezanka  1 ,  Fatima Hassouna  5
Affiliations
  • 1. Faculty of Chemical Engineering, University of Chemistry and Technology Prague, 166 28 Prague 6, Czech Republic.
  • 2. Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, 166 28 Prague 6, Czech Republic.
  • 3. Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, 166 28 Prague 6, Czech Republic. Electronic address: [email protected].
  • 4. Faculty of Biotechnology, University of Chemistry and Technology Prague, 166 28 Prague 6, Czech Republic.
  • 5. Faculty of Chemical Engineering, University of Chemistry and Technology Prague, 166 28 Prague 6, Czech Republic. Electronic address: [email protected].
PMID: 38679244 DOI: 10.1016/j.ijpharm.2024.124170
Abstract

Improving the Anticancer efficacy of chemotherapeutic drugs and photosensitizers requires innovative multifunctional nanoplatforms. This study introduces a chemo- and phototherapeutic drug delivery system (DDS) based on poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs), both PEGylated and non-PEGylated, with a mean size of 200 ± 75 nm. Colchicine (Colch) and purpurin18 (P18) were co-encapsulated into these NPs, and their in vitro drug release profiles were investigated. The Anticancer potential of these systems was evaluated across various cell lines (i.e., CaCo-2, PC-3, MCF-7, and MRC-5 cells), demonstrating enhanced NP uptake by Cancer cells compared to free drugs. Co-administration of Colch and P18 in 2D and 3D cell line models exhibited a synergistic effect, harnessing both chemotherapeutic and photodynamic effects, leading to higher Cancer cell elimination efficacy. This newly developed multifunctional DDS presents a promising platform for combined chemo- and photodynamic therapy in Cancer treatment.

Keywords
Cancer cell spheroids; Colchicine; Drug delivery system; Multifunctional nanoplatform; PEGylated PLGA nanoparticles; PLGA nanoparticles; Purpurin 18.
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