A spike virosome vaccine induces pan-sarbecovirus antibody responses in mice

  • iScience. 2024 Apr 10;27(5):109719. doi: 10.1016/j.isci.2024.109719.
Mitch Brinkkemper  1  2 ,  Meliawati Poniman  1  2 ,  Esther Siteur-van Rijnstra  3 ,  Widad Ait Iddouch  1  2 ,  Tom P L Bijl  1  2 ,  Denise Guerra  1  2 ,  Khadija Tejjani  1  2 ,  Marloes Grobben  1  2 ,  Farien Bhoelan  4 ,  Denzel Bemelman  4 ,  Ronald Kempers  4 ,  Marit J van Gils  1  2 ,  Kwinten Sliepen  1  2 ,  Toon Stegmann  4 ,  Yme U van der Velden  1  2 ,  Rogier W Sanders  1  2  5
Affiliations
  • 1. Amsterdam UMC, location University of Amsterdam, Department of Medical Microbiology and Infection Prevention, Meibergdreef 9, 1105 AZ Amsterdam, the Netherlands.
  • 2. Amsterdam institute for Infection and Immunity, Infectious diseases, Meibergdreef 9, 1105 AZ Amsterdam, the Netherlands.
  • 3. Amsterdam UMC, location University of Amsterdam, Department of Experimental Immunology, Meibergdreef 9, 1105 AZ Amsterdam, the Netherlands.
  • 4. Mymetics BV, JH Oortweg 21, CH 2333 Leiden, the Netherlands.
  • 5. Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY, USA.
PMID: 38706848 DOI: 10.1016/j.isci.2024.109719
Abstract

Zoonotic events by sarbecoviruses have sparked an epidemic (severe acute respiratory syndrome coronavirus [SARS-CoV]) and a pandemic (SARS-CoV-2) in the past two decades. The continued risk of spillovers from Animals to humans is an ongoing threat to global health and a pan-sarbecovirus vaccine would be an important contribution to pandemic preparedness. Here, we describe multivalent virosome-based vaccines that present stabilized spike proteins from four sarbecovirus strains, one from each clade. A cocktail of four monovalent virosomes or a mosaic virosome preparation induced broad sarbecovirus binding and neutralizing antibody responses in mice. Pre-existing immunity against SARS-CoV-2 and extending the intervals between immunizations enhanced antibody responses. These results should inform the development of a pan-sarbecovirus vaccine, as part of our efforts to prepare for and/or avoid a next pandemic.

Keywords
Immunology; Virology.
Products