Acrolein scavengers and detoxification: From high-throughput screening of flavonoids to mechanistic study of epigallocatechin gallate

  • J Hazard Mater. 2024 Sep 17:480:135873. doi: 10.1016/j.jhazmat.2024.135873.
Kaiyu Jiang  1 ,  Zhao Yin  2 ,  Wei Gong  3 ,  Yu-Xuan Liang  4 ,  Juncai Tu  3 ,  Xiaoya Tao  3 ,  Zhengqi Liu  3 ,  Yuanyuan Hu  3 ,  Jinjin Li  3 ,  Xiaoming Guo  3 ,  Juanying Ou  5 ,  Jie Zheng  6 ,  Beiwei Zhu  7 ,  Shiyi Ou  8
Affiliations
  • 1. Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China; Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, National, Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Shenzhen University Medical School, Shenzhen 518060, China; Shenzhen Key Laboratory of Food Nutrition and Health, College of Chemistry and Environmental Engineering and Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, China.
  • 2. Department of Hematology, Guangdong Second Provincial General Hospital, Jinan University, Guangzhou 510317, China.
  • 3. Shenzhen Key Laboratory of Food Nutrition and Health, College of Chemistry and Environmental Engineering and Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, China.
  • 4. Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, National, Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Shenzhen University Medical School, Shenzhen 518060, China; Shenzhen Key Laboratory of Food Nutrition and Health, College of Chemistry and Environmental Engineering and Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, China.
  • 5. Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China; Institute of Food Safety & Nutrition, Jinan University, Guangzhou 510632, China.
  • 6. Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.
  • 7. Shenzhen Key Laboratory of Food Nutrition and Health, College of Chemistry and Environmental Engineering and Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, China. Electronic address: [email protected].
  • 8. Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China. Electronic address: [email protected].
PMID: 39305594 DOI: 10.1016/j.jhazmat.2024.135873
Abstract

Acrolein (ACR) is a widespread, highly toxic substance that poses significant health risks. Flavonoids have been recognized as effective ACR scavengers, offering a possible way to reduce these risks. However, the lack of specific high-throughput screening methods has limited the identification of ACR scavengers, and their actual detoxifying capacity on ACR remains unknown. To address this, we developed a high-throughput screening platform to assess the ACR scavenging capacity of 322 Flavonoids. Our results showed that 80.7 % of the Flavonoids could scavenge ACR, but only 34.4 % exhibited detoxifying effects in an ACR-injured QSG7701 cell model. Some Flavonoids even increased toxicity. Structure-activity relationship (SAR) analysis indicated that galloyl and pyrogallol units enhance scavenging but worsen ACR-induced cytotoxicity. Further investigation revealed that epigallocatechin gallate (EGCG) could exacerbate ACR-induced redox disorder, leading to cell Apoptosis. Our findings provide crucial data on the scavenging and detoxifying capacities of 322 Flavonoids, highlighting that ACR scavengers might not mitigate ACR-induced toxicity and could pose additional safety risks.

Keywords
Acrolein; Detoxifying capacity; Flavonoids; High-throughput screening platform; Scavenging capacity.
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