Antisenescence Expansion of Mesenchymal Stem Cells Using Piezoelectric β-Poly(vinylidene fluoride) Film-Based Culture

  • ACS Appl Mater Interfaces. 2024 Nov 20;16(46):63207-63224. doi: 10.1021/acsami.4c12725.
Liuyue Xu  1 ,  Wenxiang Ren  2 ,  Yaoying Long  3 ,  Bianlei Yang  1 ,  Li Chen  4 ,  Wenlan Chen  3 ,  Siyi Chen  1 ,  Yulin Cao  1 ,  Di Wu  3 ,  Jiao Qu  3 ,  He Li  3 ,  Yali Yu  1 ,  Anyuan Zhang  3 ,  Shan Wang  1 ,  Hongxiang Wang  4 ,  Ting Chen  5 ,  Guifen Fan  6  7 ,  Qiubai Li  1  5 ,  Zhichao Chen  3
Affiliations
  • 1. Department of Rheumatology and Immunology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
  • 2. Center for Hematology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
  • 3. Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
  • 4. Department of Hematology, Key Laboratory for Molecular Diagnosis of Hubei Province, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China.
  • 5. Hubei Engineering Research Center for Application of Extracellular Vesicles, Hubei University of Science and Technology, Xianning 437100, China.
  • 6. School of Optical and Electronic Information, Key Lab of Functional Materials for Electronic Information(B), MOE, Huazhong University of Science and Technology, Wuhan 430074, China.
  • 7. Wenzhou Advanced Manufacturing Institute, Huazhong University of Science and Technology, Wenzhou 325035, China.
PMID: 39503875 DOI: 10.1021/acsami.4c12725
Abstract

Regenerative therapies based on mesenchymal stem cells (MSCs) show promise in treating a wide range of disorders. However, the replicative senescence of MSCs during in vitro expansion poses a challenge to obtaining a substantial quantity of high-quality MSCs. In this investigation, a piezoelectric β-poly(vinylidene fluoride) film-based culture plate (β-CP) was developed with an antisenescence effect on cultured human umbilical cord-derived MSCs. Compared to traditional tissue culture plates (TCPs) and α-poly(vinylidene fluoride) film-based culture plates, the senescence markers of p21, p53, interleukin-6 and insulin-like growth factor-binding protein-7, stemness markers of OCT4 and NANOG, and telomere length of MSCs cultured on β-CPs were significantly improved. Additionally, MSCs at passage 18 cultured on β-CPs showed significantly better multipotency and pro-angiogenic capacities in vitro, and higher wound healing abilities in a mouse model. Mechanistically, β-CPs rejuvenated senescent MSCs by improving mitochondrial functions and mitigating oxidative and glycoxidative stresses. Overall, this study presents β-CPs as a promising approach for efficient and straightforward antisenescence expansion of MSCs while preserving their stemness, thereby holding great potential for large-scale production of MSCs for clinical application in cell therapies.

Keywords
antisenescence; cell therapy; large-scale production; mesenchymal stem cells; piezoelectric PVDF films; stemness.
Products