Construction of a New Drug and Agrochemical Candidates Screening Platform Utilizing Drug-Hypersensitive Fission Yeast to Discover Overlooked Natural Products

  • J Nat Prod. 2025 Sep 26;88(9):2090-2096. doi: 10.1021/acs.jnatprod.5c00598.
Aoi Kimishima  1  2 ,  Sota Negami  1 ,  Sota Honma  1  2 ,  Shigehiro A Kawashima  3 ,  Yoko Yashiroda  4 ,  Shin-Ichi Fuji  5 ,  Hiroki Kojima  1  2 ,  Toshiyuki Tokiwa  1 ,  Akihiro Sugawara  1  2 ,  Yukiko Ujie  1  2 ,  Akio Abe  1  2 ,  Takumi Chinen  6 ,  Minoru Yoshida  4 ,  Takeo Usui  7 ,  Yukihiro Asami  1  2
Affiliations
  • 1. Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
  • 2. O̅mura Satoshi Memorial Institute, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
  • 3. Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • 4. Chemical Genomics Research Group, RIKEN Center for Sustainable Resource Science, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
  • 5. Faculty of Bioresource Sciences, Akita Prefectural University, 241-438 Kaidobata-Nishi, Nakano, Shimoshinjo Akita 010-0195, Japan.
  • 6. Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • 7. Institute of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Ibaraki 305-8572, Japan.
PMID: 40848261 DOI: 10.1021/acs.jnatprod.5c00598
Abstract

Natural products exhibiting selective or preferential Antifungal activity against fission yeast over budding yeast might contribute greatly to new discoveries in the life sciences and new drug and agrochemical development. However, it is difficult to discover new drug and agrochemical candidates in the fission yeast screening system due to its low drug sensitivity. In this study, we constructed a new Antifungal drug and agrochemical candidate screening platform using a drug-hypersensitive fission yeast strain. We executed Antifungal activity profiling of our natural compound and microbial libraries against drug-hypersensitive fission yeast and multidrug-sensitive budding yeast. Ultimately, we identified MS-347a as a promising agrochemical candidate due to its excellent activity against the rice blast fungus.

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