Differential Expression and Function of Arginase in Mouse Uterus During Early Pregnancy

The supply of Amino acids is essential to embryo survival and successful pregnancy. The accumulating evidence indicates that arginine, a semi-essential amino acid, plays a key role during early pregnancy. Arginase (ARG2) is a key enzyme for catalyzing arginine into ornithine and urea. However, the expression, regulation, and role of Arginase during early mouse pregnancy are still unknown. In our study, ARG1 immunofluorescence is mainly detected in uterine epithelium and gradually decreases from days 1 to 5 of pregnancy. From days 1 to 4 of pregnancy, there is no detectable ARG2 immunofluorescence in the mouse uterus. On day 5 of pregnancy, ARG2 signals are strongly seen in the primary decidua surrounding the implanting blastocyst at the implantation site, but not at the inter-implantation site. There is a temporary increase for ARG2 levels under mouse in vitro decidualization, suggesting ARG2 may be involved in the initiation of mouse decidualization. Prl8a2, a marker of mouse in vitro decidualization, is significantly decreased after ARG levels are suppressed. However, Arg2 overexpression obviously increases Prl8a2 levels. Mouse in vitro decidualization is downregulated by arginine and ornithine but stimulated by a low dose of urea. Urea has a beneficial effect on uterine receptivity and antioxidative Enzymes. Our results indicate that ARG2 plays an important role during mouse decidualization by balancing the levels of arginine, ornithine, and urea.