Art. -Nr.: HY-N0834

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Pulsatilla saponin D

(Synonyms: SB365; Hederacolchiside A)

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Pulsatilla saponin D (SB365), isolated from the root of Pulsatilla chinensis, is an anti-tumor agent.

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Reinheit: 99.89%
CAS. Nr.: 68027-15-6
Formel: C₄₇H₇₆O₁₇
Molecular Weight:913.10

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Pulsatilla saponin D Chemical Structure

CAS. Nr.: 68027-15-6

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Pulsatilla saponin D (SB365), isolated from the root of Pulsatilla chinensis, is an anti-tumor agent^[1]^[2]^[3]^[4].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	110 μM Compound: 26	Cell membrane permeabilization in human A549 cells assessed as drug level causing decrease in calcein fluorescence Cell membrane permeabilization in human A549 cells assessed as drug level causing decrease in calcein fluorescence	[PMID: 19200744]
A549	IC₅₀	5.8 μM Compound: 316	Antitumor activity against human A549 cells xenografted in mouse assessed as tumor growth inhibition Antitumor activity against human A549 cells xenografted in mouse assessed as tumor growth inhibition	[PMID: 37859723]
A549	IC₅₀	59 μM Compound: 26	Cytotoxicity against human A549 cells after 48 hrs by resazurin reduction test Cytotoxicity against human A549 cells after 48 hrs by resazurin reduction test	[PMID: 19200744]
A549	IC₅₀	6 μM Compound: 1	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 29131631]
A549	IC₅₀	6.3 μM Compound: 2, PSD	Cytotoxicity against human A549 cells assessed as inhibition of cell growth after 24 hrs by MTT assay Cytotoxicity against human A549 cells assessed as inhibition of cell growth after 24 hrs by MTT assay	[PMID: 25958248]
Bel-7402	IC₅₀	8.9 μM Compound: 2, PSD	Cytotoxicity against human Bel7402 cells assessed as inhibition of cell growth after 24 hrs by MTT assay Cytotoxicity against human Bel7402 cells assessed as inhibition of cell growth after 24 hrs by MTT assay	[PMID: 25958248]
DLD-1	IC₅₀	159 μM Compound: 26	Cell membrane permeabilization in human DLD1 cells assessed as drug level causing decrease in calcein fluorescence Cell membrane permeabilization in human DLD1 cells assessed as drug level causing decrease in calcein fluorescence	[PMID: 19200744]
DLD-1	IC₅₀	62 μM Compound: 26	Cytotoxicity against human DLD1 cells after 48 hrs by resazurin reduction test Cytotoxicity against human DLD1 cells after 48 hrs by resazurin reduction test	[PMID: 19200744]
HL-60	IC₅₀	3.8 μg/mL Compound: 7	Cytotoxicity against human HL-60 cells after 72 hrs by MTT assay Cytotoxicity against human HL-60 cells after 72 hrs by MTT assay	[PMID: 10514313]
L02	IC₅₀	12.6 μM Compound: 2, PSD	Cytotoxicity against human HL7702 cells assessed as inhibition of cell growth after 24 hrs by MTT assay Cytotoxicity against human HL7702 cells assessed as inhibition of cell growth after 24 hrs by MTT assay	[PMID: 25958248]
L02	IC₅₀	21.12 μM Compound: B3	Cytotoxicity against human L02 cells by MTT assay Cytotoxicity against human L02 cells by MTT assay	[PMID: 33964439]
SMMC-7721	IC₅₀	7.6 μM Compound: 2, PSD	Cytotoxicity against human SMMC7721 cells assessed as inhibition of cell growth after 24 hrs by MTT assay Cytotoxicity against human SMMC7721 cells assessed as inhibition of cell growth after 24 hrs by MTT assay	[PMID: 25958248]

In Vitro

Pulsatilla saponin D (SB365) shows apoptotic effect accompanied by increased evidence of cleaved caspase-3 and poly (ADP ribose) polymerase^[1].
Pulsatilla saponin D (SB365) strongly inhibits c-Met expression in gastric cancer cells^[1].
Pulsatilla saponin D (SB365) strongly suppresses the growth and proliferation of 5 human pancreatic cancer cell lines (MIAPaCa-2, BXPC-3, PANC-1, AsPC-1 and HPAC) ^[2].
Pulsatilla saponin D (SB365) shows anti-angiogenic activity by decreasing the expression of HIF-1α and VEGF^[3].
Pulsatilla saponin D (0.3 ng/mL to 10 μg/mL) exhibits anti-tumor activity in NSCLC cancer cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[4]

Cell Line:

A-549, SKMEL-2, MCF-7, and Lewis lung carcinoma (LLC) cells.
Concentration:

0.3 ng/mL to 10 μg/mL.
Incubation Time:

72 h.
Result:

Exhibited an ED₅₀ value of 6.3 μg/mL for A-549 cells.

In Vivo

Pulsatilla saponin D (6.4 mg/kg) exhibits anti-tumor activity in Lewis lung carcinoma model^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

2x2x2 mm³ tumor fragments of a Lewis lung carcinoma (LLC) transplanted s.c. into the auxiliary region of the BDF1 mice^[4].
Dosage:

6.4 mg/kg.
Administration:

Intraperitoneally on the 1 st to 7 th and 9 th to 14 th day.
Result:

Had such a potent antitumor effect (IR, 82 %) on a solid tumor, which is higher than Adriamycin (IR, 64%).

Chemical Information

CAS. Nr. 68027-15-6
Appearance Solid
Molecular Weight 913.10
Formel C₄₇H₇₆O₁₇
Color Off-white to light yellow
SMILES

C[C@]([C@@]1([H])[C@]2(CC[C@@H]3O[C@@](OC[C@H](O[C@]([C@@H]([C@@H](O)[C@@H]4O)O)([H])O[C@@H]4CO)[C@@H]5O)([H])[C@@H]5O[C@@](O[C@@H](C)[C@H](O)[C@H]6O)([H])[C@@H]6O)C)(CC[C@@]2([H])[C@]3(C)CO)[C@@](C7=CC1)(CC[C@]8(C(O)=O)[C@@]7([H])CC(C)(C)CC8)C
Synonyms

SB365; Hederacolchiside A
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

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Chem Biol Interact

Pulsatilla saponin D inhibited the growth of osteosarcoma by regulating the JNK/ATF3 signaling pathway. [Abstract]

2025 Apr 1:410:111420. PMID: 39954838

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : ≥ 39 mg/mL (42.71 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.0952 mL

5.4759 mL

10.9517 mL

5 mM

0.2190 mL

1.0952 mL

2.1903 mL

10 mM

0.1095 mL

0.5476 mL

1.0952 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (2.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (2.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (2.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (284 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Hong SW, et al. SB365, Pulsatilla saponin D, targets c-Met and exerts antiangiogenic and antitumor activities. Carcinogenesis. 2013 Sep;34(9):2156-69. [Content Brief]

[2]. Son MK, et al. SB365, Pulsatilla saponin D suppresses proliferation and induces apoptosis of pancreatic cancer cells. Oncol Rep. 2013 Aug;30(2):801-8. [Content Brief]

[3]. Son MK, et al. SB365, Pulsatilla saponin D suppresses the proliferation of human colon cancer cells and induces apoptosis by modulating the AKT/mTOR signalling pathway. Food Chem. 2013 Jan 1;136(1):26-33. [Content Brief]

[4]. Yong Kim, et al. Pulsatilla saponin D: the antitumor principle from Pulsatilla koreana. Arch Pharm Res. 2004 Sep;27(9):915-8. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.0952 mL	5.4759 mL	10.9517 mL	27.3793 mL
	5 mM	0.2190 mL	1.0952 mL	2.1903 mL	5.4759 mL
	10 mM	0.1095 mL	0.5476 mL	1.0952 mL	2.7379 mL
	15 mM	0.0730 mL	0.3651 mL	0.7301 mL	1.8253 mL
	20 mM	0.0548 mL	0.2738 mL	0.5476 mL	1.3690 mL
	25 mM	0.0438 mL	0.2190 mL	0.4381 mL	1.0952 mL
	30 mM	0.0365 mL	0.1825 mL	0.3651 mL	0.9126 mL
	40 mM	0.0274 mL	0.1369 mL	0.2738 mL	0.6845 mL

Keywords

Pulsatilla saponin D

1 Cited Publications

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Pulsatilla saponin D

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Publications Citing Use of MedChemExpress (MCE) Pulsatilla saponin D

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Pulsatilla saponin D

Bioactive Compound Library Max

Bioactive Compound Library

Apoptosis Compound Library

Natural Product Library

Anti-Cancer Compound Library

Glycoside Compound Library

Terpenoids Library

Traditional Chinese Medicine Active Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Cancer Natural Product Library

Plant-Sourced Natural Product Library

Cell Death Library

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Natural Product Diversity Scaffold Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Pulsatilla saponin D

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Pulsatilla saponin D Related Classifications

HY-N0834 Related Classifications