Pulsatilla saponin D
Based on 1 publication(s) in Google Scholar
Pulsatilla saponin D (SB365), isolated from the root of Pulsatilla chinensis, is an anti-tumor agent.
For research use only. We do not sell to patients.
- Purity: 99.89%
- CAS No.: 68027-15-6
- Formula: C47H76O17
- Molecular Weight:913.10
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Pulsatilla saponin D
More
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
110 μM
Compound: 26
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Cell membrane permeabilization in human A549 cells assessed as drug level causing decrease in calcein fluorescence
Cell membrane permeabilization in human A549 cells assessed as drug level causing decrease in calcein fluorescence
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[PMID: 19200744] |
| A549 | IC50 |
5.8 μM
Compound: 316
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Antitumor activity against human A549 cells xenografted in mouse assessed as tumor growth inhibition
Antitumor activity against human A549 cells xenografted in mouse assessed as tumor growth inhibition
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[PMID: 37859723] |
| A549 | IC50 |
59 μM
Compound: 26
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Cytotoxicity against human A549 cells after 48 hrs by resazurin reduction test
Cytotoxicity against human A549 cells after 48 hrs by resazurin reduction test
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[PMID: 19200744] |
| A549 | IC50 |
6 μM
Compound: 1
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Cytotoxicity against human A549 cells after 48 hrs by MTT assay
Cytotoxicity against human A549 cells after 48 hrs by MTT assay
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[PMID: 29131631] |
| A549 | IC50 |
6.3 μM
Compound: 2, PSD
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Cytotoxicity against human A549 cells assessed as inhibition of cell growth after 24 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as inhibition of cell growth after 24 hrs by MTT assay
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[PMID: 25958248] |
| Bel-7402 | IC50 |
8.9 μM
Compound: 2, PSD
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Cytotoxicity against human Bel7402 cells assessed as inhibition of cell growth after 24 hrs by MTT assay
Cytotoxicity against human Bel7402 cells assessed as inhibition of cell growth after 24 hrs by MTT assay
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[PMID: 25958248] |
| DLD-1 | IC50 |
159 μM
Compound: 26
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Cell membrane permeabilization in human DLD1 cells assessed as drug level causing decrease in calcein fluorescence
Cell membrane permeabilization in human DLD1 cells assessed as drug level causing decrease in calcein fluorescence
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[PMID: 19200744] |
| DLD-1 | IC50 |
62 μM
Compound: 26
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Cytotoxicity against human DLD1 cells after 48 hrs by resazurin reduction test
Cytotoxicity against human DLD1 cells after 48 hrs by resazurin reduction test
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[PMID: 19200744] |
| HL-60 | IC50 |
3.8 μg/mL
Compound: 7
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Cytotoxicity against human HL-60 cells after 72 hrs by MTT assay
Cytotoxicity against human HL-60 cells after 72 hrs by MTT assay
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[PMID: 10514313] |
| L02 | IC50 |
12.6 μM
Compound: 2, PSD
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Cytotoxicity against human HL7702 cells assessed as inhibition of cell growth after 24 hrs by MTT assay
Cytotoxicity against human HL7702 cells assessed as inhibition of cell growth after 24 hrs by MTT assay
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[PMID: 25958248] |
| L02 | IC50 |
21.12 μM
Compound: B3
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Cytotoxicity against human L02 cells by MTT assay
Cytotoxicity against human L02 cells by MTT assay
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[PMID: 33964439] |
| SMMC-7721 | IC50 |
7.6 μM
Compound: 2, PSD
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Cytotoxicity against human SMMC7721 cells assessed as inhibition of cell growth after 24 hrs by MTT assay
Cytotoxicity against human SMMC7721 cells assessed as inhibition of cell growth after 24 hrs by MTT assay
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[PMID: 25958248] |
Pulsatilla saponin D (SB365) shows apoptotic effect accompanied by increased evidence of cleaved caspase-3 and poly (ADP ribose) polymerase[1].
Pulsatilla saponin D (SB365) strongly inhibits c-Met expression in gastric cancer cells[1].
Pulsatilla saponin D (SB365) strongly suppresses the growth and proliferation of 5 human pancreatic cancer cell lines (MIAPaCa-2, BXPC-3, PANC-1, AsPC-1 and HPAC) [2].
Pulsatilla saponin D (SB365) shows anti-angiogenic activity by decreasing the expression of HIF-1α and VEGF[3].
Pulsatilla saponin D (0.3 ng/mL to 10 μg/mL) exhibits anti-tumor activity in NSCLC cancer cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A-549, SKMEL-2, MCF-7, and Lewis lung carcinoma (LLC) cells.
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Concentration:0.3 ng/mL to 10 μg/mL.
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Incubation Time:72 h.
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Result:Exhibited an ED50 value of 6.3 μg/mL for A-549 cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:2x2x2 mm3 tumor fragments of a Lewis lung carcinoma (LLC) transplanted s.c. into the auxiliary region of the BDF1 mice[4].
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Dosage:6.4 mg/kg.
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Administration:Intraperitoneally on the 1 st to 7 th and 9 th to 14 th day.
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Result:Had such a potent antitumor effect (IR, 82 %) on a solid tumor, which is higher than Adriamycin (IR, 64%).
Chemical Information
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CAS No. 68027-15-6
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Appearance Solid
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Molecular Weight 913.10
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Formula C47H76O17
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Color Off-white to light yellow
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SMILES
C[C@]([C@@]1([H])[C@]2(CC[C@@H]3O[C@@](OC[C@H](O[C@]([C@@H]([C@@H](O)[C@@H]4O)O)([H])O[C@@H]4CO)[C@@H]5O)([H])[C@@H]5O[C@@](O[C@@H](C)[C@H](O)[C@H]6O)([H])[C@@H]6O)C)(CC[C@@]2([H])[C@]3(C)CO)[C@@](C7=CC1)(CC[C@]8(C(O)=O)[C@@]7([H])CC(C)(C)CC8)C
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Synonyms
SB365; Hederacolchiside A
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Chem Biol Interact
Pulsatilla saponin D inhibited the growth of osteosarcoma by regulating the JNK/ATF3 signaling pathway. [Abstract]2025 Apr 1:410:111420. PMID: 39954838
Solvent & Solubility
DMSO : ≥ 39 mg/mL (42.71 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (2.28 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (2.28 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (284 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Hong SW, et al. SB365, Pulsatilla saponin D, targets c-Met and exerts antiangiogenic and antitumor activities. Carcinogenesis. 2013 Sep;34(9):2156-69. [Content Brief]
[2]. Son MK, et al. SB365, Pulsatilla saponin D suppresses proliferation and induces apoptosis of pancreatic cancer cells. Oncol Rep. 2013 Aug;30(2):801-8. [Content Brief]
[3]. Son MK, et al. SB365, Pulsatilla saponin D suppresses the proliferation of human colon cancer cells and induces apoptosis by modulating the AKT/mTOR signalling pathway. Food Chem. 2013 Jan 1;136(1):26-33. [Content Brief]
[4]. Yong Kim, et al. Pulsatilla saponin D: the antitumor principle from Pulsatilla koreana. Arch Pharm Res. 2004 Sep;27(9):915-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.0952 mL | 5.4759 mL | 10.9517 mL | 27.3793 mL |
| 5 mM | 0.2190 mL | 1.0952 mL | 2.1903 mL | 5.4759 mL | |
| 10 mM | 0.1095 mL | 0.5476 mL | 1.0952 mL | 2.7379 mL | |
| 15 mM | 0.0730 mL | 0.3651 mL | 0.7301 mL | 1.8253 mL | |
| 20 mM | 0.0548 mL | 0.2738 mL | 0.5476 mL | 1.3690 mL | |
| 25 mM | 0.0438 mL | 0.2190 mL | 0.4381 mL | 1.0952 mL | |
| 30 mM | 0.0365 mL | 0.1825 mL | 0.3651 mL | 0.9126 mL | |
| 40 mM | 0.0274 mL | 0.1369 mL | 0.2738 mL | 0.6845 mL |
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