1. Apoptosis
  2. Apoptosis
  3. Pulsatilla saponin D

Pulsatilla saponin D (Synonyms: SB365; Hederacolchiside A)

Cat. No.: HY-N0834 Purity: 98.47%
Handling Instructions

Pulsatilla saponin D (SB365), isolated from the root of Pulsatilla koreana Nakai, is an anti-tumor agent.

For research use only. We do not sell to patients.

Pulsatilla saponin D Chemical Structure

Pulsatilla saponin D Chemical Structure

CAS No. : 68027-15-6

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Solution
10 mM * 1 mL in DMSO USD 627 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 627 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 312 In-stock
Estimated Time of Arrival: December 31
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Description

Pulsatilla saponin D (SB365), isolated from the root of Pulsatilla koreana Nakai, is an anti-tumor agent[1][2][3][4].

In Vitro

Pulsatilla saponin D (SB365) shows apoptotic effect accompanied by increased evidence of cleaved caspase-3 and poly (ADP ribose) polymerase[1].
Pulsatilla saponin D (SB365) strongly inhibits c-Met expression in gastric cancer cells[1].
Pulsatilla saponin D (SB365) strongly suppresses the growth and proliferation of 5 human pancreatic cancer cell lines (MIAPaCa-2, BXPC-3, PANC-1, AsPC-1 and HPAC) [2].
Pulsatilla saponin D (SB365) shows anti-angiogenic activity by decreasing the expression of HIF-1α and VEGF[3].
Pulsatilla saponin D (0.3 ng/mL to 10 μg/mL) exhibits anti-tumor activity in NSCLC cancer cells[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay[4]

Cell Line: A-549, SKMEL-2, MCF-7, and Lewis lung carcinoma (LLC) cells.
Concentration: 0.3 ng/mL to 10 μg/mL.
Incubation Time: 72 h.
Result: Exhibited an ED50 value of 6.3 μg/mL for A-549 cells.
In Vivo

Pulsatilla saponin D (6.4 mg/kg) exhibits anti-tumor activity in Lewis lung carcinoma model[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 2x2x2 mm3 tumor fragments of a Lewis lung carcinoma (LLC) transplanted s.c. into the auxiliary region of the BDF1 mice[4].
Dosage: 6.4 mg/kg.
Administration: Intraperitoneally on the 1 st to 7 th and 9 th to 14 th day.
Result: Had such a potent antitumor effect (IR, 82 %) on a solid tumor, which is higher than Adriamycin (IR, 64%).
Molecular Weight

913.10

Formula

C47H76O17

CAS No.
SMILES
Structure Classification
Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 39 mg/mL (42.71 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0952 mL 5.4759 mL 10.9517 mL
5 mM 0.2190 mL 1.0952 mL 2.1903 mL
10 mM 0.1095 mL 0.5476 mL 1.0952 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (2.28 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (2.28 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (2.28 mM); Clear solution

*All of the co-solvents are available by MCE.
References
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Pulsatilla saponin D
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