1. JAK/STAT Signaling
  2. Pim
  3. R8-T198wt

R8-T198wt 

Cat. No.: HY-P1404
Handling Instructions

R8-T198wt is a cell-permeable carboxyl-terminal p27Kip1 peptide exhibits anti-tumor activity by inhibiting Pim-1 kinase.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

R8-T198wt Chemical Structure

R8-T198wt Chemical Structure

CAS No. : 2305815-72-7

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Description

R8-T198wt is a cell-permeable carboxyl-terminal p27Kip1 peptide exhibits anti-tumor activity by inhibiting Pim-1 kinase.

In Vitro

R8-T198wt peptide (KD=323 nM) shows significant affinity for Pim-1. R8-T198wt peptide inhibits p27Kip1 phosphorylation by Pim-1 in cells[1].
R8-T198wt peptide induces G1 arrest and apoptosis in DU145 cells[1].
R8-T198wt treatment also enhances the HA-p27Kip1 expression level[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

2820.33

Formula

C₁₁₁H₂₁₁N₅₉O₂₆S

CAS No.

2305815-72-7

Sequence Shortening

GGGRRRRRRRRGCKKPGLRRRQT

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
References
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The dilution calculator equation

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This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
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Keywords:

R8-T198wtPimPim kinasesInhibitorinhibitorinhibit

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Product Name:
R8-T198wt
Cat. No.:
HY-P1404
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