CD36 Protein, Mouse (HEK293, His-Fc)

CD36 is a multifunctional glycoprotein that binds to proteins such as thrombospondin, fibronectin, collagen, beta-amyloid and oxidized low-density lipoprotein, as well as lipid molecules such as anionic phospholipids and long-chain phospholipids. CD36 regulates angiogenesis, inflammatory responses, fatty acid metabolism, taste, dietary fat processing, and acts as a TLR4:TLR6 heterodimer coreceptor to mediate inflammatory responses, regulate immune responses and bacterial absorption. CD36 Protein, Mouse (HEK293, His-Fc) is the recombinant mouse-derived CD36 protein, expressed by HEK293 , with C-hFc, C-His labeled tag.

CD36, a multifunctional glycoprotein, serves as a receptor for a diverse range of ligands, encompassing proteinaceous entities like thrombospondin, fibronectin, collagen, amyloid-beta, and lipidic molecules such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids, and bacterial diacylated lipopeptides. The engagement of these ligands initiates signal transduction and internalization of receptor-ligand complexes, with responses varying in a ligand-specific manner. CD36's involvement spans angiogenesis, inflammatory responses, fatty acid metabolism, taste perception, and dietary fat processing in the intestine. The glycoprotein binds long-chain fatty acids, facilitating their cellular transport and participating in processes such as muscle lipid utilization, adipose energy storage, and gut fat absorption. Mechanistically, fatty acid binding activates downstream kinase LYN, resulting in CD36 depalmitoylation and caveolar endocytosis. CD36 also plays a pivotal role in oral fat perception, influencing preferences and leading to changes in pancreatobiliary secretions upon the detection of long-chain fatty acids in the tongue. Furthermore, it contributes to the regulation of energy and glucose homeostasis in the ventromedial hypothalamus and acts as a receptor for thrombospondins, mediating antiangiogenic effects. Acting as a coreceptor for TLR4:TLR6 heterodimer, CD36 promotes inflammation in monocytes/macrophages, responding to ligands like oxLDL or amyloid-beta. Additionally, CD36 acts as a selective sensor for microbial diacylated lipopeptides, triggering NF-kappa-B-dependent cytokine production and participating in the response to infections, including M. tuberculosis and Plasmodium falciparum. It also mediates the uptake of certain bacteria, showcasing its role as a versatile and indispensable component in various physiological processes.

Mouse

HEK293

C-hFc;C-His

Q08857 (G30-K439)

12491  [NCBI]

Extracellular Domain

GDMLIEKTIKREVVLEEGTTAFKNWVKTGTTVYRQFWIFDVQNPDDVAKNSSKIKVKQRGPYTYRVRYLAKENITQDPEDHTVSFVQPNGAIFEPSLSVGTEDDNFTVLNLAVAAAPHIYQNSFVQVVLNSLIKKSKSSMFQTRSLKELLWGYKDPFLSLVPYPISTTVGVFYPYNDTVDGVYKVFNGKDNISKVAIIESYKGKRNLSYWPSYCDMINGTDAASFPPFVEKSRTLRFFSSDICRSIYAVFGSEIDLKGIPVYRFVLPANAFASPLQNPDNHCFCTEKVISNNCTSYGVLDIGKCKEGKPVYISLPHFLHASPDVSEPIEGLHPNEDEHRTYLDVEPITGFTLQFAKRLQVNILVKPARKIEALKNLKRPYIVPILWLNETGTIGDEKAEMFKTQVTGKIK

Approximately 110-120 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

≥ 85%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.