SIRP alpha/CD172a Protein, Human (CAA71403, HEK293, His)
Based on 1 Customer Validation
SIRP alpha/CD172a Protein, Human (CAA71403.1, HEK 293, His) , as a recombinant protein produced in HEK293 cells, is a polypeptide chain with a His tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
SIRP alpha/CD172a Protein, Human (CAA71403.1, HEK 293, His) , as a recombinant protein produced in HEK293 cells, is a polypeptide chain with a His tag.
Background
SIRPA is a specific cell-surface marker for isolating cardiomyocytes derived from human pluripotent stem cells[1].
Verified Bioactivity
1.Human CD47 (HY-P7335) immobilized on CM5 Chip can bind SIRP alpha/CD172a with an affinity constant of 44.32 nM as determined in SPR assay.
2.Loaded Anti-Human SIRPA mAb-Fc on Pro-A Biosensor, can bind Recombinant Human SIRPA with an affinity constant of 20.1 nM as determined in BLI assay.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
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Bioactivity - SPR
Bioactivity - SPR
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
CAA71403.1 (E31-N370)
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Molecular Construction
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N-term
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CD172a (E31-N370)
Accession # CAA71403.1 -
6*His
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C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
SIRPA; MYD1; Prev. PTPNS1; Brain-Immunoglobulin-Like Molecule With Tyrosine-Based Activation Motifs; SHPS1; Brain Ig-Like Molecule With Tyrosine-Based Activation Motifs; SIRP; Protein Tyrosine Phosphatase, Non-Receptor Type Substrate 1; BIT; Tyrosine Phos
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AA Sequence
EEELQVIQPDKSVSVAAGESAILHCTVTSLIPVGPIQWFRGAGPARELIYNQKEGHFPRVTTVSESTKRENMDFSISISNITPADAGTYYCVKFRKGSPDTEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNERN
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Predicted Molecular Mass
38.1 kDa
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Molecular Weight
Approximately 45-65 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (263 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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