Latent TGF beta 1/TGFB1 Protein, Human (HEK293, His)
Based on 1 Customer Validation
TGF beta 1/TGFB1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. TGF beta 1 is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, apoptosis, and can regulate the expression and activation of other growth factors, including interferon gamma and tumor necrosis factor alpha. Latent TGF beta 1/TGFB1 Protein, Human (HEK293, His) is the recombinant human-derived Latent TGF beta 1/TGFB1 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
TGF beta 1/TGFB1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. TGF beta 1 is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, apoptosis, and can regulate the expression and activation of other growth factors, including interferon gamma and tumor necrosis factor alpha. Latent TGF beta 1/TGFB1 Protein, Human (HEK293, His) is the recombinant human-derived Latent TGF beta 1/TGFB1 protein, expressed by HEK293 , with C-His labeled tag.
Background
Transforming growth factor (TGF) beta 1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. TGF beta 1 is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, apoptosis, and can regulate the expression and activation of other growth factors, including interferon gamma and tumor necrosis factor alpha. In humans, TGF-β1 is encoded by the TGFB1 gene. TGF beta 1 activates CREB3L1 by regulating intramembranous proteolysis, stimulating sustained collagen production. TGF beta 1 mediates SMAD2/3 activation by inducing SMAD2/3 phosphorylation and subsequent translocation to the nucleus and regulates dental papilla cells by promoting IPO7-mediated translocation of phosphorylated SMAD2 to the nucleus and subsequent transcription of target genes. TGF beta 1 induces epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types.TGF beta 1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages[1][2][3][4][5][6].
Verified Bioactivity
Immobilized Human TGF beta 1 His at 2 μg/ml (100 μl/well) can bind Human TGFBR2 His & hFc, the EC50 of Human TGFBR2 His & hFc is 5-37.2 ng/mL.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
P01137 (L30-S390)
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Molecular Construction
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N-term
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TGFB1 (L30-S390)
Accession # P01137 -
His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
TGFB1; Transforming Growth Factor Beta1; Prev. TGFB; Camurati-Engelmann Disease; Prev. DPD1; Latency-Associated Peptide; TGFbeta; TGF-Beta-1; CED; TGF-Beta1; Transforming Growth Factor Beta-1 Proprotein; IBDIMDE; Prepro-Transforming Growth Factor Beta-1;
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AA Sequence
LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS
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Predicted Molecular Mass
42.4 kDa
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Molecular Weight
Approximately 16 kDa (TGFβ1) & 38 kDa (LAP) & 55 kDa (Latent TGFβ1) under reduced (R) conditions, 110 kDa under non reducing (N) conditions, based on SDS-PAGE, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (266 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[2]. Vaughn SP, et al. Confirmation of the mapping of the Camurati-Englemann locus to 19q13. 2 and refinement to a 3.2-cM region. Genomics. 2000 May 15;66(1):119-21. [Content Brief]
[3]. Chen Q, et al. Sustained induction of collagen synthesis by TGF-β requires regulated intramembrane proteolysis of CREB3L1. PLoS One. 2014 Oct 13;9(10):e108528. [Content Brief]
[4]. Kotlarz D, et al. Human TGF-β1 deficiency causes severe inflammatory bowel disease and encephalopathy. Nat Genet. 2018 Mar;50(3):344-348. [Content Brief]
[5]. Hwangbo C, et al. Syntenin regulates TGF-β1-induced Smad activation and the epithelial-to-mesenchymal transition by inhibiting caveolin-mediated TGF-β type I receptor internalization. Oncogene. 2016 Jan 21;35(3):389-401. [Content Brief]
[6]. Letterio JJ, rt al. Regulation of immune responses by TGF-beta. Annu Rev Immunol. 1998;16:137-61. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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