TrkA Protein, Human (HEK293)
Based on 1 publication(s) in Google Scholar
TrkA Protein, Human (HEK293) is the high affinity catalytic receptor for the neurotrophin, nerve growth factor (NGF), and mediates the multiple effects of NGF, which include neuronal differentiation and avoidance of programmed cell death.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
TrkA Protein, Human (HEK293) is the high affinity catalytic receptor for the neurotrophin, nerve growth factor (NGF), and mediates the multiple effects of NGF, which include neuronal differentiation and avoidance of programmed cell death.
Background
Tyrosine kinase receptor A (TrkA) is the high affinity catalytic receptor for the neurotrophin, nerve growth factor (NGF), and mediates the multiple effects of NGF, which include neuronal differentiation and avoidance of programmed cell death. TrkA is originally cloned from a colon tumor; the cancer occurred via a translocation, which results in the activation of the TrkA kinase domain[1][2].
Verified Bioactivity
1.This product does not contain protein kinase domain.
2.The ED50 is <1 μg/mL as measured by a neutralization assay in the presence of 10.0 ng/ml human β-NGF using TF-1 Cells.
3.Measured by its binding ability in a functional ELISA. Immobilized Human TrkA at 1 μg/mL (100 μl/well) can bind Human Beta-NGF (HY-P700018AF). The ED50 for this effect is 162.8 ng/mL.
Publications (1)
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Journal Impact Factor
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Most Recent
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Am J Physiol Lung Cell Mol Physiol
2024 Aug 1;327(2):L160-L172. PMID: 38771132
Technical Parameters
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Species Human
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Source HEK293
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Tag Tag Free
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Accession
P04629-1 (A34-P407)
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Molecular Construction
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N-term
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TrkA (A34-P407)
Accession # P04629-1 -
C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
NTRK1; Tyrosine Kinase Receptor A; Neurotrophic Receptor Tyrosine Kinase 1; P140-TrkA; TRKA; Gp140trk; TRK; Trk-A; MTC; Neurotrophic Tyrosine Kinase Receptor Type 1; High Affinity Nerve Growth Factor Receptor; Tyrosine-Protein Kinase Receptor; Neurotrophic Tyrosine Kinase, Receptor, Type 1; Tyrosine Kinase Receptor; TRK1-Transforming Tyrosine Kinase Protein; Oncogene TRK; Tropomyosin Receptor Kinase A; TRK1; Tropomyosin-Related Kinase A
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AA Sequence
APCPDACCPHGSSGLRCTRDGALDSLHHLPGAENLTELYIENQQHLQHLELRDLRGLGELRNLTIVKSGLRFVAPDAFHFTPRLSRLNLSFNALESLSWKTVQGLSLQELVLSGNPLHCSCALRWLQRWEEEGLGGVPEQKLQCHGQGPLAHMPNASCGVPTLKVQVPNASVDVGDDVLLRCQVEGRGLEQAGWILTELEQSATVMKSGGLPSLGLTLANVTSDLNRKNVTCWAENDVGRAEVSVQVNVSFPASVQLHTAVEMHHWCIPFSVDGQPAPSLRWLFNGSVLNETSFIFTEFLEPAANETVRHGCLRLNQPTHVNNGNYTLLAANPFGQASASIMAAFMDNPFEFNPEDPIPVSFSPVDTNSTSGDP
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Molecular Weight
Approximately 65-85 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS.
<0.2 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (264 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Benito-Gutiérrez E, et al. Origin and evolution of the Trk family of neurotrophic receptors. Mol Cell Neurosci. 2006 Feb;31(2):179-92. [Content Brief]
[2]. Lambiase A, et al. Molecular basis for keratoconus: lack of TrkA expression and its transcriptional repression by Sp3. Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16795-800. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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