RECQL5-IN-1
Based on 1 Customer Validation
RECQL5-IN-1 (Compound 4a) acts as an orally effective RECQL5 inhibitor (targeting both enzymatic and nonenzymatic domain). RECQL5-IN-1 is a potent inhibitor of RECQL5 helicase activity (IC50=46.3 nM), stabilizes the interaction between RECQL5-RAD51 proteins, causes RAD51 aggregation and homologous recombination repair (HRR) inhibition, thereby exhibiting selective cytotoxicity in RECQL5-expressing cancer cells.
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- Pureza : 98.40%
- No. CAS: 2718170-45-5
- Fòrmula: C25H18F6N4O2S
- Peso molecular:552.49
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Descripciòn
IC50 & Target
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Helicase |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MCF7 | IC50 |
19.6 μM
Compound: 4a
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Cytotoxicity against RECQL5 knock out human MCF7 cells assessed as inhibition of colony formation measured after 8 to 10 days by clonogenic survival assay
Cytotoxicity against RECQL5 knock out human MCF7 cells assessed as inhibition of colony formation measured after 8 to 10 days by clonogenic survival assay
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[PMID: 33529023] |
| MCF7 | IC50 |
4.8 μM
Compound: 4a
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Cytotoxicity against human MCF7 cells expressing wild type RECQL5 assessed as inhibition of colony formation measured after 8 to 10 days by clonogenic survival assay
Cytotoxicity against human MCF7 cells expressing wild type RECQL5 assessed as inhibition of colony formation measured after 8 to 10 days by clonogenic survival assay
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[PMID: 33529023] |
In Vitro
RECQL5-IN-1 (0-50 μM, 24-72 h) causes the accumulation of double-strand breaks (DSBs), inhibits MCF-7 and MCF10A with IC20s of 8.4 μM and 33.4 μM. RECQL5-IN-1 inhibits the colony formation of RECQL5-wildtype cell[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7 and MCF10A
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Concentration:0-50 μM
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Incubation Time:72 h
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Result:Inhibited the cell viability.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:MCF-7 xenograft mouse models[1]
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Dosage:50 mg/kg
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Administration:po, every two days for 30 days
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Result:Inhibited tumor growth.
Chemical Information
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No. CAS 2718170-45-5
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Appearance Solid
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Peso molecular 552.49
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Fòrmula C25H18F6N4O2S
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Color White to off-white
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SMILES
FC(F)(F)C1=CC(C(F)(F)F)=CC(NC(N[C@@H](C2=CC=CC=C2)C3=NN=C(SCC4=CC=CC=C4)O3)=O)=C1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 250 mg/mL (452.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (278 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8100 mL | 9.0499 mL | 18.0999 mL | 45.2497 mL |
| 5 mM | 0.3620 mL | 1.8100 mL | 3.6200 mL | 9.0499 mL | |
| 10 mM | 0.1810 mL | 0.9050 mL | 1.8100 mL | 4.5250 mL | |
| 15 mM | 0.1207 mL | 0.6033 mL | 1.2067 mL | 3.0166 mL | |
| 20 mM | 0.0905 mL | 0.4525 mL | 0.9050 mL | 2.2625 mL | |
| 25 mM | 0.0724 mL | 0.3620 mL | 0.7240 mL | 1.8100 mL | |
| 30 mM | 0.0603 mL | 0.3017 mL | 0.6033 mL | 1.5083 mL | |
| 40 mM | 0.0452 mL | 0.2262 mL | 0.4525 mL | 1.1312 mL | |
| 50 mM | 0.0362 mL | 0.1810 mL | 0.3620 mL | 0.9050 mL | |
| 60 mM | 0.0302 mL | 0.1508 mL | 0.3017 mL | 0.7542 mL | |
| 80 mM | 0.0226 mL | 0.1131 mL | 0.2262 mL | 0.5656 mL | |
| 100 mM | 0.0181 mL | 0.0905 mL | 0.1810 mL | 0.4525 mL |
RECQL5-IN-1 Related Classifications
HY-145685 Related Classifications
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