REF1 peptide
Based on 1 Customer Validation
REF1 peptide is a PORK1 agonist with an EC50 of 0.028 nM in tomato. REF1 peptide binds to the extracellular domain of PORK1, triggers receptor autophosphorylation, and drives downstream MPK3/MPK6 activation, thereby initiating defense and regeneration signaling cascades. REF1 peptide induces callus formation, inhibits shoot regeneration upon continuous exposure, and enhances the regeneration and transformation efficiency of recalcitrant dicotyledonous and monocotyledonous crops.
For research use only. We do not sell to patients.
- Purity: 99.95%
- CAS No.: 1198397-79-3
- Formula: C101H169N39O32
- Molecular Weight:2441.66
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
REF1 peptide (100 nM; 12 h) potently induces the expression of the PI-II defense gene in wild-type Solanum lycopersicum seedlings[1].
REF1 peptide (1 nM; 7-14 d) restores the callus formation and shoot regeneration defects of hypocotyl explants from Solanum lycopersicum prp-2, prp-3 and spr9 mutants[1].
REF1 peptide (100 nM; 30 min) induces the activation of MPK3/6 in Solanum lycopersicum seedlings in a PORK1 receptor-dependent manner[1].
REF1 peptide (2 μg/mL; 24 h) increases the p53-mediated transcriptional activation levels of p21, Bax and GADD45 promoters by approximately 2-fold in H1299 cells[2].
REF1 peptide (20 μg/10-cm plate; 12-72 h) increases p53-induced endogenous p21 mRNA levels and elevates cyclin G mRNA levels in H1299 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:H1299 tetracycline-inducible p53 cell lines
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Concentration:pHook2-REF1 peptide vector (20 μg/10-cm plate)
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Incubation Time:72 h (transfection); 12 h (tetracycline maintenance/removal)
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Result:Further increased p53-induced p21 mRNA by ~40%.
Enhanced p53-induced cyclin G mRNA increase (2-fold without REF1 peptide) by 50-100%.
Had no effect on p21 or cyclin G mRNA in the absence of p53.
Chemical Information
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CAS No. 1198397-79-3
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Appearance Solid
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Molecular Weight 2441.66
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Formula C101H169N39O32
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Color White to off-white
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Sequence
Ala-Thr-Asp-Arg-Arg-Gly-Arg-Pro-Pro-Ser-Arg-Pro-Lys-Val-Gly-Ser-Gly-Pro-Pro-Pro-Gln-Asn-Asn
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Sequence Shortening
ATDRRGRPPSRPKVGSGPPPQNN
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : ≥ 100 mg/mL (40.96 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (279 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.4096 mL | 2.0478 mL | 4.0956 mL | 10.2389 mL |
| 5 mM | 0.0819 mL | 0.4096 mL | 0.8191 mL | 2.0478 mL | |
| 10 mM | 0.0410 mL | 0.2048 mL | 0.4096 mL | 1.0239 mL | |
| 15 mM | 0.0273 mL | 0.1365 mL | 0.2730 mL | 0.6826 mL | |
| 20 mM | 0.0205 mL | 0.1024 mL | 0.2048 mL | 0.5119 mL | |
| 25 mM | 0.0164 mL | 0.0819 mL | 0.1638 mL | 0.4096 mL | |
| 30 mM | 0.0137 mL | 0.0683 mL | 0.1365 mL | 0.3413 mL | |
| 40 mM | 0.0102 mL | 0.0512 mL | 0.1024 mL | 0.2560 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
REF1 peptide Related Classifications
HY-P11056 Related Classifications
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