2. Apoptosis
  3. MDM-2/p53
  4. REF1 peptide

REF1 peptide is a PORK1 agonist with an EC50 of 0.028 nM in tomato. REF1 peptide binds to the extracellular domain of PORK1, triggers receptor autophosphorylation, and drives downstream MPK3/MPK6 activation, thereby initiating defense and regeneration signaling cascades. REF1 peptide induces callus formation, inhibits shoot regeneration upon continuous exposure, and enhances the regeneration and transformation efficiency of recalcitrant dicotyledonous and monocotyledonous crops.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

REF1 peptide

REF1 peptide Chemical Structure

CAS No. : 1198397-79-3

Size Price Stock Quantity
1 mg In-stock
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 Customer Validation

REF1 peptide Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

REF1 peptide is a PORK1 agonist with an EC50 of 0.028 nM in tomato. REF1 peptide binds to the extracellular domain of PORK1, triggers receptor autophosphorylation, and drives downstream MPK3/MPK6 activation, thereby initiating defense and regeneration signaling cascades. REF1 peptide induces callus formation, inhibits shoot regeneration upon continuous exposure, and enhances the regeneration and transformation efficiency of recalcitrant dicotyledonous and monocotyledonous crops[1][2].

In Vitro

REF1 peptide (100 nM; 12 h) potently induces the expression of the PI-II defense gene in wild-type Solanum lycopersicum seedlings[1].
REF1 peptide (1 nM; 7-14 d) restores the callus formation and shoot regeneration defects of hypocotyl explants from Solanum lycopersicum prp-2, prp-3 and spr9 mutants[1].
REF1 peptide (100 nM; 30 min) induces the activation of MPK3/6 in Solanum lycopersicum seedlings in a PORK1 receptor-dependent manner[1].
REF1 peptide (2 μg/mL; 24 h) increases the p53-mediated transcriptional activation levels of p21, Bax and GADD45 promoters by approximately 2-fold in H1299 cells[2].
REF1 peptide (20 μg/10-cm plate; 12-72 h) increases p53-induced endogenous p21 mRNA levels and elevates cyclin G mRNA levels in H1299 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

REF1 peptide Related Antibodies

RT-PCR[2]

Cell Line: H1299 tetracycline-inducible p53 cell lines
Concentration: pHook2-REF1 peptide vector (20 μg/10-cm plate)
Incubation Time: 72 h (transfection); 12 h (tetracycline maintenance/removal)
Result: Further increased p53-induced p21 mRNA by ~40%.
Enhanced p53-induced cyclin G mRNA increase (2-fold without REF1 peptide) by 50-100%.
Had no effect on p21 or cyclin G mRNA in the absence of p53.
Molecular Weight

2441.66

Formula

C101H169N39O32
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Ala-Thr-Asp-Arg-Arg-Gly-Arg-Pro-Pro-Ser-Arg-Pro-Lys-Val-Gly-Ser-Gly-Pro-Pro-Pro-Gln-Asn-Asn
Sequence Shortening

ATDRRGRPPSRPKVGSGPPPQNN
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (40.96 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.4096 mL 2.0478 mL 4.0956 mL
5 mM 0.0819 mL 0.4096 mL 0.8191 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.4096 mL 2.0478 mL 4.0956 mL 10.2389 mL
5 mM 0.0819 mL 0.4096 mL 0.8191 mL 2.0478 mL
10 mM 0.0410 mL 0.2048 mL 0.4096 mL 1.0239 mL
15 mM 0.0273 mL 0.1365 mL 0.2730 mL 0.6826 mL
20 mM 0.0205 mL 0.1024 mL 0.2048 mL 0.5119 mL
25 mM 0.0164 mL 0.0819 mL 0.1638 mL 0.4096 mL
30 mM 0.0137 mL 0.0683 mL 0.1365 mL 0.3413 mL
40 mM 0.0102 mL 0.0512 mL 0.1024 mL 0.2560 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

REF1 peptide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

REF1 peptide1198397-79-3MDM-2/p53RKO cellsMPK3/6SlWIND1PORK1H1299 cellsSolanum habrochaitesArabidopsis thalianaSolanum lycopersicumSolanum peruvianump53Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
REF1 peptide
Cat. No.:
HY-P11056
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (254 KB) Français - FR (254 KB) Deutsch - DE (254 KB) Norwegian - NO (254 KB) Español - ES (254 KB) Swedish - SV (254 KB) Italian - IT (254 KB) Korean - KR (254 KB) Portuguese - PT (254 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.