Based on 1 Customer Validation
RepSox (E-616452) (GMP) is a RepSox (HY-13012) produced by using GMP guidelines. GMP small molecules works appropriately as an auxiliary reagent for cell therapy manufacture. RepSox is a potent and selective TGF-β-RI/ALK5 inhibitor.
For research use only. We do not sell to patients.
- CAS No.: 446859-33-2
- Formula: C17H13N5
- Molecular Weight:287.32
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
IC50: ALK5 autophosphorylation (4 nM)
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HepG2 | IC50 |
0.01 μM
Compound: 19
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Stimulation of Transforming growth factor beta receptor I kinase in HepG2 cells
Stimulation of Transforming growth factor beta receptor I kinase in HepG2 cells
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[PMID: 15317461] |
| K562 | IC50 |
>100 μM
Compound: ALK5 inhibitor II
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Cytotoxicity against human K562 cells assessed as decrease in cell proliferation after 72 hrs by calcein AM staining-based assay
Cytotoxicity against human K562 cells assessed as decrease in cell proliferation after 72 hrs by calcein AM staining-based assay
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[PMID: 28135685] |
| MCF7 | IC50 |
>100 μM
Compound: ALK5 inhibitor II
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Cytotoxicity against human MCF7 cells assessed as decrease in cell proliferation after 72 hrs by calcein AM staining-based assay
Cytotoxicity against human MCF7 cells assessed as decrease in cell proliferation after 72 hrs by calcein AM staining-based assay
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[PMID: 28135685] |
| Sf9 | IC50 |
0.004 μM
Compound: 19
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Inhibition of recombinant Activin A receptor type II-like kinase (ALK5) expressed in baculovirus/Sf9 cells
Inhibition of recombinant Activin A receptor type II-like kinase (ALK5) expressed in baculovirus/Sf9 cells
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[PMID: 15317461] |
RepSox (GMP) (10 μM) induces MEFs differentiating to chemically induced pluripotent stem cells (CiPSCs)[1].
RepSox (GMP) (10 μM, in stage I-Stage III induction medium) induces hCiPS cells from HEFs[2].
RepSox (GMP) together with Forskolin (HY-15371) increases the number of proliferative cells in MEFs expressing MyoD, confirmed by increased EdU incorporation[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 446859-33-2
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Appearance Solid
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Molecular Weight 287.32
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Formula C17H13N5
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Color White to yellow
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SMILES
CC1=NC(C2=NNC=C2C3=NC4=CC=CN=C4C=C3)=CC=C1
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Synonyms
E-616452 (GMP); SJN 2511 (GMP)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Zhao T, et al. Single-Cell RNA-Seq Reveals Dynamic Early Embryonic-like Programs during Chemical Reprogramming. Cell Stem Cell. 2018 Jul 5;23(1):31-45.e7. [Content Brief]
[2]. Guan J, et al. Chemical reprogramming of human somatic cells to pluripotent stem cells. Nature. 2022 May;605(7909):325-331. [Content Brief]
[3]. Bar-Nur O, et al. Direct Reprogramming of Mouse Fibroblasts into Functional Skeletal Muscle Progenitors. Stem Cell Reports. 2018 May 8;10(5):1505-1521. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
RepSox (GMP) Related Classifications
HY-13012G Related Classifications
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