Rhodamine-DHPE
Rhodamine-DHPE is a fluorescently labeled phosphatidylethanolamine lipid that labels phospholipid bilayers. Rhodamine-DHPE serves as a fluorescence quenching substrate and membrane stain. The fluorescence lifetime of Rhodamine-DHPE decreases significantly in the presence of Cu2+-PS complexes. Rhodamine-DHPE effectively stains the membranes of human red blood cells and mouse fibroblasts, and supports lifetime-resolved imaging via pump-probe fluorescence microscopy.
For research use only. We do not sell to patients.
- CAS No.: 126111-99-7
- Formula: C70H117N4O14PS2
- Molecular Weight:1333.80
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Cu2+-PS complexes dynamically quench the fluorescence of Rhodamine-DHPE in supported lipid bilayers, with reduced fluorescence lifetime and temperature-independent quenching efficiency[2].
Rhodamine-DHPE (5 mg/mL; 30 min) is used in imaging human erythrocytes, where pump-probe fluorescence microscopy provides superior spatial resolution and off-focal background rejection for imaging Rhodamine-DHPE-labeled human erythrocytes compared to conventional one-photon microscopy[3].
Rhodamine-DHPE (10 μg/mL; 30 min) is used in imaging fixed mouse fibroblast cells, where pump-probe fluorescence microscopy provides superior spatial resolution for imaging Rhodamine-DHPE-labeled fixed mouse fibroblast cells compared to conventional one-photon microscopy[3].
Rhodamine-DHPE (10 μg/mL; 30 min) is used in imaging fixed mouse fibroblast cells, where lifetime-resolved pump-probe fluorescence microscopy distinguishes Rhodamine-DHPE-stained cytoplasmic structures (lifetime of 2.0 ns) from ethidium bromide-labeled nuclei in fixed mouse fibroblast cells, generating clear contrast between regions with overlapping emission spectra[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 126111-99-7
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Molecular Weight 1333.80
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Formula C70H117N4O14PS2
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SMILES
CCN(CC)CC.O=S([O-])(C1=C(C=CC(S(NCCOP(OC[C@H](OC(CCCCCCCCCCCCCCC)=O)COC(CCCCCCCCCCCCCCC)=O)(O)=O)(=O)=O)=C1)C2=C(C=CC(N(CC)CC)=C3)C3=[O+]C4=C2C=CC(N(CC)CC)=C4)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Simonsson L, et al. Continuous lipid bilayers derived from cell membranes for spatial molecular manipulation. J Am Chem Soc. 2011;133(35):14027-14032. [Content Brief]
[2]. Poyton M F. Metallomembranes: Characterizing Transition Metal Ion-Lipid Complexes[J]. 2016.
[3]. Dong CY, et al. Fluorescence lifetime imaging by asynchronous pump-probe microscopy. Biophys J. 1995;69(6):2234-2242. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Rhodamine-DHPE Related Classifications
HY-203233 Related Classifications
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- Rhodamine-DHPE
- 126111-99-7
- Fluorescent Dye
- membrane stain
- phosphatidylethanolamine lipid
- lipid bilayer marker
- human erythrocyte
- artificial high-cholesterol lipid vesicles
- 3T3 fibroblast cell membrane-derived vesicles
- pump-probe fluorescence microscopy
- POPC SLB
- mouse fibroblast cell membranes
- fluorescence quenching substrate
- Inhibitor
- inhibitor
- inhibit