Rhodamine-DHPE 

Rhodamine-DHPE is a fluorescently labeled phosphatidylethanolamine lipid that labels phospholipid bilayers. Rhodamine-DHPE serves as a fluorescence quenching substrate and membrane stain. The fluorescence lifetime of Rhodamine-DHPE decreases significantly in the presence of Cu²⁺-PS complexes. Rhodamine-DHPE effectively stains the membranes of human red blood cells and mouse fibroblasts, and supports lifetime-resolved imaging via pump-probe fluorescence microscopy^[1][2][3].

Cu²⁺-PS complexes dynamically quench the fluorescence of Rhodamine-DHPE in supported lipid bilayers, with reduced fluorescence lifetime and temperature-independent quenching efficiency^[2].
Rhodamine-DHPE (5 mg/mL; 30 min) is used in imaging human erythrocytes, where pump-probe fluorescence microscopy provides superior spatial resolution and off-focal background rejection for imaging Rhodamine-DHPE-labeled human erythrocytes compared to conventional one-photon microscopy^[3].
Rhodamine-DHPE (10 μg/mL; 30 min) is used in imaging fixed mouse fibroblast cells, where pump-probe fluorescence microscopy provides superior spatial resolution for imaging Rhodamine-DHPE-labeled fixed mouse fibroblast cells compared to conventional one-photon microscopy^[3].
Rhodamine-DHPE (10 μg/mL; 30 min) is used in imaging fixed mouse fibroblast cells, where lifetime-resolved pump-probe fluorescence microscopy distinguishes Rhodamine-DHPE-stained cytoplasmic structures (lifetime of 2.0 ns) from ethidium bromide-labeled nuclei in fixed mouse fibroblast cells, generating clear contrast between regions with overlapping emission spectra^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1333.80

C₇₀H₁₁₇N₄O₁₄PS₂

126111-99-7

CCN(CC)CC.O=S([O-])(C1=C(C=CC(S(NCCOP(OC[C@H](OC(CCCCCCCCCCCCCCC)=O)COC(CCCCCCCCCCCCCCC)=O)(O)=O)(=O)=O)=C1)C2=C(C=CC(N(CC)CC)=C3)C3=[O+]C4=C2C=CC(N(CC)CC)=C4)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Simonsson L, et al. Continuous lipid bilayers derived from cell membranes for spatial molecular manipulation. J Am Chem Soc. 2011;133(35):14027-14032.  [Content Brief]

  [2]. Poyton M F. Metallomembranes: Characterizing Transition Metal Ion-Lipid Complexes[J]. 2016.

  [3]. Dong CY, et al. Fluorescence lifetime imaging by asynchronous pump-probe microscopy. Biophys J. 1995;69(6):2234-2242.