2. Cell Cycle/DNA Damage
  3. DNA/RNA Synthesis
  4. RNase L-IN-1

RNase L-IN-1 is a RNase L inhibitor with an IC50 of 15.9 μM. RNase L-IN-1 binds to the ATP-binding pocket of RNase L, and inhibits ribosomal RNA cleavage. RNase L-IN-1 can be used for the research of RNase L-associated cellular processes.

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RNase L-IN-1

RNase L-IN-1 화학구조

사이즈 가격 재고 수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 해외재고보유
Solution
10 mM * 1 mL in DMSO 해외재고보유
Solid
1 mg 해외재고보유
5 mg 해외재고보유
10 mg 해외재고보유
25 mg 해외재고보유
50 mg 해외재고보유
100 mg 해외재고보유
200 mg   견적 받기  
500 mg   견적 받기  

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 Customer Validation

Other Forms of RNase L-IN-1:

RNase L-IN-1 Related Antibodies

Top Publications Citing Use of Products

View All DNA/RNA Synthesis Isoform Specific Products:

View All Isoforms
RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

  • Biological Activity

  • 순도&문서

  • References

  • 고객리뷰

제품 설명

RNase L-IN-1 is a RNase L inhibitor with an IC50 of 15.9 μM. RNase L-IN-1 binds to the ATP-binding pocket of RNase L, and inhibits ribosomal RNA cleavage. RNase L-IN-1 can be used for the research of RNase L-associated cellular processes[1].

IC50 & Target[1]

RNASE L

15.9 μM (IC50)

In Vitro

RNase L-IN-1 (compound 17a) (4-250 μM; 30-30 min) completely inhibits purified human full-length RNase L-mediated RNA cleavage at 250 μM, with clear dose-dependent activity at lower concentrations[1].
RNase L-IN-1 (1.56-100 μM; 400-1200 s) exhibits significantly concentration-dependent binding to biotinylated purified human full-length RNase L[1].
RNase L-IN-1 (2-10 μM; 4 h) efficiently inhibits poly I:C-induced rRNA cleavage in HeLa cells, with almost complete inhibition at 10 μM[1].
RNase L-IN-1 binds to the ATP-binding pocket of human RNase L with improved shape complementarity and forms multiple hydrogen bonds with key residues (Lys392, Gln489, Glu404), contributing to its enhanced inhibitory potency[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RNase L-IN-1 Related Antibodies
분자량

542.67

화학식

C27H35FN6O3S
Appearance

Solid

Color

Yellow to brown

SMILES

O=C1/C(SC(NC2=CC(F)=CC=C2)=C1C(NCCN)=O)=C\C3=C(C)C(C(NCCN(CC)CC)=O)=C(C)N3
선적

Room temperature in continental US; may vary elsewhere.
보관
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
용액&용해도
In Vitro: 

DMSO : 8.33 mg/mL (15.35 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8427 mL 9.2137 mL 18.4274 mL
5 mM 0.3685 mL 1.8427 mL 3.6855 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • 몰농도 계산기

  • 농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 0.83 mg/mL (1.53 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 0.83 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8427 mL 9.2137 mL 18.4274 mL 46.0685 mL
5 mM 0.3685 mL 1.8427 mL 3.6855 mL 9.2137 mL
10 mM 0.1843 mL 0.9214 mL 1.8427 mL 4.6069 mL
15 mM 0.1228 mL 0.6142 mL 1.2285 mL 3.0712 mL
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RNase L-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RNase L-IN-1DNA/RNA Synthesisribosomal RNA cleavageGlu404RNase Lpoly I:CATP-binding pocketRibonuclease LrRNA cleavageLys392Gln489HeLa cellsInhibitorinhibitorinhibit

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Inquiry Information

상품명:
RNase L-IN-1
Cat. No.:
HY-155583
수량:
MCE Japan Authorized Agent:

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