ROX azide, 5-isomer
ROX azide, 5-isomer is a red-emitting rhodamine dye possessing high brightness and fluorescence quantum yield. The azide group can react with alkyne, BCN, DBCO via Click Chemistry to yield a stable triazole linkage.
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- CAS No.: 2628213-67-0
- Formule: C36H36N6O4
- Masse moléculaire:616.71
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
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Activité biologique
Description
Chemical Information
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CAS No. 2628213-67-0
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Masse moléculaire 616.71
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Formule C36H36N6O4
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SMILES
[N-]=[N+]=NCCCNC(C1=CC(C([O-])=O)=C(C(C2=C(O3)C(CCCN4CCC5)=C4C5=C2)=C(C=C6CCC7)C3=C8C6=[N+]7CCC8)C=C1)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Pureté et documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
ROX azide, 5-isomer Related Classifications
HY-W800808 Related Classifications
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