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Cat. No.: HY-D1760

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Sodium-binding benzofuran isophthalate

SBFI is a cell-impermeant, fluorescent Na⁺ indicator dye. SBFI is excited at 340 nm and the fluorophore emission is collected at 450 nm. SBFI selective for Na⁺ over K⁺ with K_d values of 20 and 120 mM for these ions, respectively. .

For research use only. We do not sell to patients.

CAS No.: 124549-08-2
Formula: C₄₄H₄₂N₂O₁₅
Molecular Weight:838.81

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (272 KB)

Sodium-binding benzofuran isophthalate Chemical Structure

CAS No.: 124549-08-2

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
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Biological Activity

Description

SBFI is a cell-impermeant, fluorescent Na⁺ indicator dye. SBFI is excited at 340 nm and the fluorophore emission is collected at 450 nm^[1]. SBFI selective for Na⁺ over K⁺ with K_d values of 20 and 120 mM for these ions, respectively. ^[2].

In Vitro

The SBFI dye for each sample was prepared by : ^[1]
1. dissolving the appropriate quantity of dye powder in 3 mL of dimethyl sulphoxide.
2. The five solutions were agitated for 5 minutes.
3. Each sample was then exposed to ultraviolet light with a Leitz Orthoplan microscope, and fluorescence levels recorded.
4. Ten readings in total were achieved for each solution, agitating between readings.
5. For each set of sample results the highest, lowest, and median values were plotted against dye concentration.
6. From these tests the optimum fluorescence values, with minimum variation of upper and lower limits, was established with 50 g of SBFI dye.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 124549-08-2
Molecular Weight 838.81
Formula C₄₄H₄₂N₂O₁₅
SMILES

O=C(C1=CC=C(C(C(O)=O)=C1)C2=CC3=CC(OC)=C(N4CCOCCOCCN(CCOCC4)C5=C(OC)C=C6C=C(C7=CC=C(C(O)=O)C=C7C(O)=O)OC6=C5)C=C3O2)O
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Leeves MA, The effect of mechanical deformation on the distribution of ions in fibroblasts. Am J Orthod Dentofacial Orthop. 1995 Jun;107(6):625-32. [Content Brief]

[2]. Minta A, Fluorescent indicators for cytosolic sodium. J Biol Chem. 1989 Nov 15;264(32):19449-57. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Sodium-binding benzofuran isophthalate

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Biological Activity
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Purity & Documentation
References

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Sodium-binding benzofuran isophthalate

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

Sodium-binding benzofuran isophthalate Related Classifications

HY-D1760 Related Classifications