Cat. No.: HY-N4314

Scutellarein tetramethyl ether

(Synonyms: 4',5,6,7-Tetramethoxyflavone)

5 Cited Publications

Customer Review

Based on 5 publication(s) in Google Scholar

Scutellarein tetramethyl ether (4',5,6,7-Tetramethoxyflavone) is a bioactive compound extracted from Eupatorium odoratum. Scutellarein tetramethyl ether exhibits anti-inflammatory, antibacterial, pro-coagulant, and anti-tumor activities. Scutellarein tetramethyl ether exerts its anti-inflammatory effects by modulating the NF-κB pathway and regulates bacterial resistance through the inhibition of efflux pumps. Additionally, Scutellarein tetramethyl ether accelerates coagulation time via the endogenous coagulation pathway. Studies have shown that Scutellarein tetramethyl ether can effectively inhibit the growth of the liver cancer cell line HepG2 (IC₅₀= 20.08 μg/mL).

For research use only. We do not sell to patients.

Purity: 99.64%
CAS No.: 1168-42-9
Formula: C₁₉H₁₈O₆
Molecular Weight:342.34

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Scutellarein tetramethyl ether Chemical Structure

CAS No.: 1168-42-9

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Publications (5)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All COX Isoforms :

All NO Synthase Isoforms :

All NF-κB Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HT-1080	ED₅₀	4.5 μg/mL Compound: b	Antiproliferative activity against human HT1080 cells after 4 days by MTT assay Antiproliferative activity against human HT1080 cells after 4 days by MTT assay	[PMID: 11374950]
K562	IC₅₀	42 μM Compound: 24	Inhibitory activity against K562 human chronic myelogenous leukemia cell line using MTT assay after 5 days incubation of the compound Inhibitory activity against K562 human chronic myelogenous leukemia cell line using MTT assay after 5 days incubation of the compound	[PMID: 14552774]
KB	ED₅₀	27 μg/mL Compound: NSC-53908	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 469554]
MDA-MB-231	EC₅₀	292.3 μM Compound: 21	Cytotoxicity against human MDA-MB-231 cells Cytotoxicity against human MDA-MB-231 cells	[PMID: 28112509]
Monocyte	IC₅₀	22 μM Compound: Tetra-O-methylscutellarein	Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA	[PMID: 10096854]

In Vitro

Scutellarein tetramethyl ether (50 and 100 μM; 1 h) inhibits the expression of COX-2 and iNOS in RAW 264.7 cells induced by LPS (HY-D1056)^[1].
Scutellarein tetramethyl ether (10-100 μM; 1 h) reduces the activity of NF-κB promoter in transiently transfected RAW 264.7 cells in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

Transient transfection of RAW 264.7 cells
Concentration:

10, 50 and 100 µM
Incubation Time:

1 h
Result:

Suppressed both phospho-IKKα/β and phospho-IκBα expression at concentrations of 50 and 100 µM.

Chemical Information

CAS No. 1168-42-9
Appearance Solid
Molecular Weight 342.34
Formula C₁₉H₁₈O₆
Color Off-white to light yellow
SMILES

O=C1C=C(C2=CC=C(OC)C=C2)OC3=CC(OC)=C(OC)C(OC)=C13
Synonyms

4',5,6,7-Tetramethoxyflavone
Structure Classification
- Flavonoids
- Flavones
Initial Source
- Plants
- Compositae
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (5)

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Journal Impact Factor

Nat Commun

Discovery of senolytics using machine learning. [Abstract]

2023 Jun 10;14(1):3445. PMID: 37301862
Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 Oct 15:489:144992. PMID: 40466530
Ind Crops Prod

2026 May 8;246:123392.
Ind Crops Prod

2025 Dec 11;239:122458.

Solvent & Solubility

In Vitro:

DMSO : 83.33 mg/mL (243.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.9211 mL

14.6054 mL

29.2107 mL

5 mM

0.5842 mL

2.9211 mL

5.8421 mL

10 mM

0.2921 mL

1.4605 mL

2.9211 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (6.08 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (6.08 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (6.08 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Pandith H, et al. Effect of Siam weed extract and its bioactive component scutellarein tetramethyl ether on anti-inflammatory activity through NF-κB pathway. J Ethnopharmacol. 2013 May 20;147(2):434-41. [Content Brief]

[2]. Maia GL, et al. Flavonoids from Praxelis clematidea R.M. King and Robinson modulate bacterial drug resistance. Molecules. 2011 Jun 10;16(6):4828-35. [Content Brief]

[3]. Triratana T, et al. Effect of Eupatorium odoratum on blood coagulation. J Med Assoc Thai. 1991 May;74(5):283-7. [Content Brief]

[4]. Xianfeng Zhang, et al. "Dissecting the antitumor effects of Scutellaria barbata: Initial insights into the metabolism of scutellarin and luteolin by gut microbiota." Journal of Pharmaceutical and Biomedical Analysis 248 (2024): 116325. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.9211 mL	14.6054 mL	29.2107 mL	73.0268 mL
	5 mM	0.5842 mL	2.9211 mL	5.8421 mL	14.6054 mL
	10 mM	0.2921 mL	1.4605 mL	2.9211 mL	7.3027 mL
	15 mM	0.1947 mL	0.9737 mL	1.9474 mL	4.8685 mL
	20 mM	0.1461 mL	0.7303 mL	1.4605 mL	3.6513 mL
	25 mM	0.1168 mL	0.5842 mL	1.1684 mL	2.9211 mL
	30 mM	0.0974 mL	0.4868 mL	0.9737 mL	2.4342 mL
	40 mM	0.0730 mL	0.3651 mL	0.7303 mL	1.8257 mL
	50 mM	0.0584 mL	0.2921 mL	0.5842 mL	1.4605 mL
	60 mM	0.0487 mL	0.2434 mL	0.4868 mL	1.2171 mL
	80 mM	0.0365 mL	0.1826 mL	0.3651 mL	0.9128 mL
	100 mM	0.0292 mL	0.1461 mL	0.2921 mL	0.7303 mL

Keywords

Scutellarein tetramethyl ether

5 Cited Publications

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Scutellarein tetramethyl ether

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Publications Citing Use of MedChemExpress (MCE) Scutellarein tetramethyl ether

All COX Isoforms

All NO Synthase Isoforms

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (5)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Scutellarein tetramethyl ether Related Classifications

HY-N4314 Related Classifications