BT18
Based on 1 publication(s) in Google Scholar
BT18 is a molecule mimic with function similar to glial cell line-derived neurotrophic factor (GDNF). BT18 shows an effect on GDNF family receptor GFRα1 and RET receptor tyrosine kinase RetA function.
For research use only. We do not sell to patients.
- Purity: 99.77%
- CAS No.: 924811-53-0
- Formula: C30H31F4N3O6S
- Molecular Weight:637.64
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) BT18
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Biological Activity
BT18 (50 μM; 1-90 minutes) activates AKT and ERK1/2 in time-dependently in MG87RET cell lines[1].
BT18 (1-50 μM; 90 minutes) activates AKT in a dose-dependent manner in MG87RET cell lines[1].
BT18 (5-50 μM; 15 mimutes) appears to phosphorylate RET in concentration of 50 µM both in GFP-transfected and in GFRD1-transfected cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MG87RET cell lines
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Concentration:50 μM
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Incubation Time:1, 5, 15, 45, 90 minutes
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Result:Activated AKT and ERK1/2 in time-dependently.
Chemical Information
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CAS No. 924811-53-0
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Appearance Solid
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Molecular Weight 637.64
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Formula C30H31F4N3O6S
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Color White to light yellow
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SMILES
O=C(N1CCN(C2=CC(S(=O)(N3CC4=C(C=C(OC)C(OC)=C4)CC3)=O)=CC=C2OC)CC1)C5=CC=C(F)C=C5C(F)(F)F
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
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Journal Impact Factor
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Most Recent
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Cell Oncol (Dordr)
Tumor-associated macrophage-derived GDNF promotes gastric cancer liver metastasis via a GFRA1-modulated autophagy flux. [Abstract]2023 Apr;46(2):315-330. PMID: 36808605
Solvent & Solubility
DMSO : 100 mg/mL (156.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.92 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5683 mL | 7.8414 mL | 15.6828 mL | 39.2071 mL |
| 5 mM | 0.3137 mL | 1.5683 mL | 3.1366 mL | 7.8414 mL | |
| 10 mM | 0.1568 mL | 0.7841 mL | 1.5683 mL | 3.9207 mL | |
| 15 mM | 0.1046 mL | 0.5228 mL | 1.0455 mL | 2.6138 mL | |
| 20 mM | 0.0784 mL | 0.3921 mL | 0.7841 mL | 1.9604 mL | |
| 25 mM | 0.0627 mL | 0.3137 mL | 0.6273 mL | 1.5683 mL | |
| 30 mM | 0.0523 mL | 0.2614 mL | 0.5228 mL | 1.3069 mL | |
| 40 mM | 0.0392 mL | 0.1960 mL | 0.3921 mL | 0.9802 mL | |
| 50 mM | 0.0314 mL | 0.1568 mL | 0.3137 mL | 0.7841 mL | |
| 60 mM | 0.0261 mL | 0.1307 mL | 0.2614 mL | 0.6535 mL | |
| 80 mM | 0.0196 mL | 0.0980 mL | 0.1960 mL | 0.4901 mL | |
| 100 mM | 0.0157 mL | 0.0784 mL | 0.1568 mL | 0.3921 mL |
BT18 Related Classifications
HY-111969 Related Classifications
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