Sophoramine
Based on 1 publication(s) in Google Scholar
Sophoramine ((-)-Sophoramine) is a prejunctional α2-adrenoceptor inhibitor that can be found in the seeds of Sophora alopecuroides. Sophoramine inhibits prejunctional α2-adrenoceptors to facilitate norepinephrine release from adrenergic nerves. Sophoramine shows cytotoxic activity against human cancer cells, positive inotropic activity, and activity in rodent arrhythmia models. Sophoramine can be used for the research of arrhythmias.
For research use only. We do not sell to patients.
- Purity : 99%
- CAS No.: 6882-66-2
- Formula: C15H20N2O
- Molecular Weight:244.33
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Sophoramine
MoreAll Adrenergic Receptor Isoforms
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Biological Activity
Description
IC50 & Target
[1]|
α2-adrenergic receptor |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
>40 μM
Compound: 4
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Antiproliferative activity against human A549 cells after 3 days by SRB assay
Antiproliferative activity against human A549 cells after 3 days by SRB assay
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[PMID: 26865176] |
| KB | IC50 |
>40 μM
Compound: 4
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Antiproliferative activity against human KB cells after 3 days by SRB assay
Antiproliferative activity against human KB cells after 3 days by SRB assay
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[PMID: 26865176] |
| MDA-MB-231 | IC50 |
>40 μM
Compound: 4
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Antiproliferative activity against human MDA-MB-231 cells after 3 days by SRB assay
Antiproliferative activity against human MDA-MB-231 cells after 3 days by SRB assay
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[PMID: 26865176] |
| MDCK | IC50 |
63.07 μM
Compound: 17
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Antiviral activity against influenza A virus (A/Hanfang/359/95(H3N2)) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenic effect dosed 1 hr after viral adsorption
Antiviral activity against influenza A virus (A/Hanfang/359/95(H3N2)) infected in MDCK cells assessed as inhibition of virus-induced cytopathogenic effect dosed 1 hr after viral adsorption
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[PMID: 26132528] |
In Vitro
Sophoramine (10-4-10-3 M) produces a concentration-dependent relaxation in dog vessels and coronary[2].
Sophoramine (10-4 M) potentiates the contractile response to ransmuraf stimulation in dog mesenteric arteries and veins[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 6882-66-2
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Appearance Solid
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Molecular Weight 244.33
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Formula C15H20N2O
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Color Light yellow to yellow
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SMILES
O=C1N2C([C@]3([H])[C@]4([H])[C@](CCCN4CCC3)([H])C2)=CC=C1
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Synonyms
(-)-Sophoramine
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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Phytomedicine
Compound Kushen injection improves M1 macrophage polarisation and radiation-induced colitis by regulating Piezo1-mediated NF-κB/NLRP3 pathway. [Abstract]2026 May:154:158055. PMID: 41818947
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cardiac voltage-sensitive optical mapping
Cardiac voltage-sensitive optical mapping records changes in transmembrane potential from cardiac tissue by staining the preparation with a voltage-sensitive dye and imaging fluorescence changes during electrical activation; the resulting optical action potentials can be used to map activation time, action potential duration, conduction velocity, wavefront propagation, and arrhythmia dynamics. The optical signal represents a relative fluorescence change from a tissue volume rather than a single-cell intracellular recording, so spatial resolution, sampling rate, voltage resolution, optical magnification, light penetration, and motion control must be considered together when interpreting optical action potentials.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Rong ZJ, et al. A novel alkaloid from the seeds of Sophora alopecuroides L. Nat Prod Res. 2022 Apr;36(7):1864-1869. [Content Brief]
[2]. Bian K, et al. Effects of sophoramine, an alkaloid from Sophora alopecuroides on isolated dog blood vessels. J Ethnopharmacol. 1988;24(2-3):167-178. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Sophoramine Related Classifications
HY-N3198A Related Classifications
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