SGK1-IN-4
Based on 2 publication(s) in Google Scholar
SGK1-IN-4 (compound 17a) is a highly selective, orally active SGK1 inhibitor. SGK1-IN-4 can be used for osteoarthritis research.
For research use only. We do not sell to patients.
- Purity: 99.80%
- CAS No.: 1628048-93-0
- Formula: C23H21ClFN5O4S
- Molecular Weight:517.96
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Orally Active Compound Library
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Publications Citing Use of MedChemExpress (MCE) SGK1-IN-4
More
Biological Activity
IC50: 3 nM (human SGK1, ATP=500 μM), 253 nM (mouse SGK1, ATP=500 μM), 358 nM (rat SGK1, ATP=500 μM)[1]
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Cell Line
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Type | Value | Description | References |
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| U2OS | IC50 |
0.12 μM
Compound: 79
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Inhibition of recombinant SGK1 (unknown origin) expressed in U2OS cells by cellular activity assay
Inhibition of recombinant SGK1 (unknown origin) expressed in U2OS cells by cellular activity assay
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[PMID: 25589922] |
SGK1-IN-4 inhibits human SGK1, mouse SGK1 and rat SGK1 (with an ATP concentration of 500 μM) with IC50s of 3 nM, 253 nM and 358 nM, respectively. SGK1-IN-4 inhibits the hypertrophic differentiation of chondrocytes in ATDC5 with an IC50 of 50 nM[1].
SGK1-IN-4 (2-10 μM, 2 weeks) reduces the expression of chondrocyte hypertrophy markers such as collagen type X, exhibits a smoother surface, inhibits the hypertrophic differentiation of chondrocytes in mouse isolated femoral head cartilage[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1628048-93-0
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Appearance Solid
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Molecular Weight 517.96
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Formula C23H21ClFN5O4S
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Color White to light yellow
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SMILES
O=S(NC(C=C1)=CC=C1C2=NC(O[C@@H]3CC[C@H](CC3)O)=C4C(NN=C4)=N2)(C5=C(C=CC(Cl)=C5)F)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Arch Virol
Serum/glucocorticoid-regulated kinase 1 contributes to the proliferation of varicella-zoster virus and induction of cyclin B1 expression. [Abstract]2024 May 9;169(5):116. PMID: 38722402 -
Solvent & Solubility
DMSO : ≥ 100 mg/mL (193.07 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.02 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (4.02 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9307 mL | 9.6533 mL | 19.3065 mL | 48.2663 mL |
| 5 mM | 0.3861 mL | 1.9307 mL | 3.8613 mL | 9.6533 mL | |
| 10 mM | 0.1931 mL | 0.9653 mL | 1.9307 mL | 4.8266 mL | |
| 15 mM | 0.1287 mL | 0.6436 mL | 1.2871 mL | 3.2178 mL | |
| 20 mM | 0.0965 mL | 0.4827 mL | 0.9653 mL | 2.4133 mL | |
| 25 mM | 0.0772 mL | 0.3861 mL | 0.7723 mL | 1.9307 mL | |
| 30 mM | 0.0644 mL | 0.3218 mL | 0.6436 mL | 1.6089 mL | |
| 40 mM | 0.0483 mL | 0.2413 mL | 0.4827 mL | 1.2067 mL | |
| 50 mM | 0.0386 mL | 0.1931 mL | 0.3861 mL | 0.9653 mL | |
| 60 mM | 0.0322 mL | 0.1609 mL | 0.3218 mL | 0.8044 mL | |
| 80 mM | 0.0241 mL | 0.1207 mL | 0.2413 mL | 0.6033 mL | |
| 100 mM | 0.0193 mL | 0.0965 mL | 0.1931 mL | 0.4827 mL |
SGK1-IN-4 Related Classifications
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