SPC5001

Cat. No.: HY-148687: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

SPC5001 is a locked nucleic acid (LNA)-modifed antisense oligonucleotide (ASO) complementary to human PCSK9 (proprotein convertase subtilisin/kexin type 9) mRNA. SPC5001 can be used for the research of hypercholesterolemia. SPC5001 sequence: 5′-TGmCTACAAAACmCmCA-3′.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (SPC5001 sodium) that retains the same biological activity.

For research use only. We do not sell to patients.

5′-TGmCTACAAAACmCmCA-3′

SPC5001 Chemical Structure

Size	Price	Stock
1 mg	USD 534	Ask For Quote & Lead Time
5 mg	USD 1400	Ask For Quote & Lead Time
10 mg	USD 2380	Ask For Quote & Lead Time

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

SPC5001 (0.16-40 µM; 72 h) induces the downregulation of PCSK9 mRNA targets but cytotoxicity is not observed at any concentration in 2D human renal proximal tubule epithelial cells (HRPTEC) cultures^[1].
SPC5001 (5 µM; 48 h or 20 d) downregulates PCSK9 mRNA in chip-cultured HRPTEC, with 74.1% and 93.7% for 48 h and 20 days, respectively^[1].
SPC5001 (5 µM; 20 d) induces cytotoxicity and increases perfusate levels of biomarkers KIM-1, NGAL, clusterin, osteopontin and VEGF in chip-cultured HRPTEC^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

4689.85

Appearance

Solid

Color

White to off-white

SMILES

[SPC5001]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (21.32 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.2132 mL	1.0661 mL	2.1323 mL
5 mM	0.0426 mL	0.2132 mL	0.4265 mL
10 mM	0.0213 mL	0.1066 mL	0.2132 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 93.97%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (246 KB) RP-HPLC (219 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Nieskens TTG, et, al. Nephrotoxic antisense oligonucleotide SPC5001 induces kidney injury biomarkers in a proximal tubule-on-a-chip. Arch Toxicol. 2021 Jun;95(6):2123-2136. [Content Brief]

[2]. Lindholm MW, Elmén J, Fisker N, et al. PCSK9 LNA antisense oligonucleotides induce sustained reduction of LDL cholesterol in nonhuman primates. Mol Ther. 2012;20(2):376-381. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	0.2132 mL	1.0661 mL	2.1323 mL	5.3307 mL
	5 mM	0.0426 mL	0.2132 mL	0.4265 mL	1.0661 mL
	10 mM	0.0213 mL	0.1066 mL	0.2132 mL	0.5331 mL
	15 mM	0.0142 mL	0.0711 mL	0.1422 mL	0.3554 mL
	20 mM	0.0107 mL	0.0533 mL	0.1066 mL	0.2665 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SPC5001SPC 5001SPC-5001OthersPCSK9ntisense oligonucleotideASOmRNAhypercholesterolemia5′-TGmCTACAAAACmCmCA-3′Inhibitorinhibitorinhibit

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