2. Anti-infection
  3. Bacterial
  4. SppIP

SppIP  (Synonyms: Sakacin P inducer peptide)

Cat. No.: HY-P11146 Purity: 98.99%
COA
SDS
Handling Instructions Technical Support

SppIP (Sakacin P inducer peptide) is a peptide consisting of 19 amino acids. SppIP is an essential inducer for sakacin P production. SppIP can induce the expression of PRRSV GP5 and SARS-CoV-2 spike protein in recombinant Lactobacillus plantarum. SppIP can activate the transcription of the sakacin P promoter to drive the expression of downstream heterologous capsid proteins, increasing the proportion of PCV2d capsid proteins displayed on the cell surface of Lactobacillus plantarum. SppIP can be used in studies related to sakacin P induction.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

SppIP

SppIP Chemical Structure

CAS No. : 175483-64-4

Size Price Stock Quantity
1 mg In-stock
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

SppIP Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

SppIP (Sakacin P inducer peptide) is a peptide consisting of 19 amino acids. SppIP is an essential inducer for sakacin P production. SppIP can induce the expression of PRRSV GP5 and SARS-CoV-2 spike protein in recombinant Lactobacillus plantarum. SppIP can activate the transcription of the sakacin P promoter to drive the expression of downstream heterologous capsid proteins, increasing the proportion of PCV2d capsid proteins displayed on the cell surface of Lactobacillus plantarum. SppIP can be used in studies related to sakacin P induction[1][2][3][4].

In Vitro

SppIP (10-200 ng/mL; 4-8 h) induces expression of PRRSV GP5 in recombinant Lactobacillus plantarum CGMCC 1.557, with maximum production achieved using 200 ng/mL SppIP for 7 h at 33 °C in the pSIP-1320-O5MH strain, and expression remains stable across 5 bacterial passages[2].
SppIP (50 ng/mL; 8 h) induces expression of SARS-CoV-2 spike protein in Lactobacillus plantarum Lp18:S that is stable after 20 min at 50 °C or 30 min at pH = 1.5, and shows increased expression after 2 h exposure to 0.2% bile salt[3].
SppIP (50 ng/mL; 3-12 h) induces time-dependent expression and surface localization of PCV2d His6-tag-capsid protein in recombinant Lactobacillus plantarum pLp3050-His6-tag-capsid, with maximal production (4.8 μg per 1010 CFU) in cell wall fractions after 12 h, while also reducing bacterial growth rate[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SppIP Related Antibodies

Western Blot Analysis[3]

Cell Line: recombinant Lactobacillus plantarum Lp18:S strain
Concentration: 50 ng/mL
Incubation Time: 8 h
Result: Induced expression of SARS-CoV-2 spike protein.

Western Blot Analysis[4]

Cell Line: recombinant Lactobacillus plantarum pLp3050-His6-tag-capsid
Concentration: 50 ng/mL
Incubation Time: 3 h
Result: Detected ~25 kDa His6-tag-capsid protein in cell-free supernatants and ~28 kDa protein (signal peptide + His6-tag-capsid) in cell wall fractions of recombinant L.
plantarum after 3 h induction; no such bands were seen in non-induced cells or control L.
plantarum pLp3050 cells.
In Vivo

SppIP (50 ng/mL; in vitro induction; 12 h) drives PCV2d capsid protein expression in recombinant L. plantarum, which when orally administered to mice elicits a significant mucosal immune response with capsid-specific sIgA production and PCV2d neutralizing activity[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c (female, 6-week-old)[4]
Dosage: 50 ng/mL (added to bacterial cultures for induction)
Administration: in vitro induction; 12 h
Result: Produced 4.8 μg PCV2d capsid protein per 1010 CFU of recombinant L.
plantarum in the cell wall fraction.
Produced 1.6 μg PCV2d capsid protein per 1010 CFU of recombinant L.
plantarum in the cell-free supernatant.
Elicited a significant increase in fecal capsid-specific sIgA levels.
Induced fecal virus neutralizing activity with a 50% reduction in PCV2d infectivity at a dilution factor of 1:55.
Molecular Weight

2229.56

Formula

C99H157N31O26S
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Met-Ala-Gly-Asn-Ser-Ser-Asn-Phe-Ile-His-Lys-Ile-Lys-Gln-Ile-Phe-Thr-His-Arg
Sequence Shortening

MAGNSSNFIHKIKQIFTHR
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (44.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 7.14 mg/mL (3.20 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.4485 mL 2.2426 mL 4.4852 mL
5 mM 0.0897 mL 0.4485 mL 0.8970 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 0.4485 mL 2.2426 mL 4.4852 mL 11.2130 mL
DMSO 5 mM 0.0897 mL 0.4485 mL 0.8970 mL 2.2426 mL
10 mM 0.0449 mL 0.2243 mL 0.4485 mL 1.1213 mL
15 mM 0.0299 mL 0.1495 mL 0.2990 mL 0.7475 mL
20 mM 0.0224 mL 0.1121 mL 0.2243 mL 0.5606 mL
25 mM 0.0179 mL 0.0897 mL 0.1794 mL 0.4485 mL
30 mM 0.0150 mL 0.0748 mL 0.1495 mL 0.3738 mL
40 mM 0.0112 mL 0.0561 mL 0.1121 mL 0.2803 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

SppIP Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SppIP175483-64-4Sakacin P inducer peptideBacterialporcine reproductive and respiratory syndromesakPporcine reproductive and respiratory syndrome virus glycoprotein 5porcine circovirus type 2d infectionsakacin P structural geneLactobacillus plantarumPCV2d capsid proteinSARS-CoV-2 spike proteinLactobacillus sake LTH673lactic acid bacteriapeptideInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
SppIP
Cat. No.:
HY-P11146
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (252 KB) Français - FR (252 KB) Deutsch - DE (252 KB) Norwegian - NO (252 KB) Español - ES (252 KB) Swedish - SV (252 KB) Italian - IT (252 KB) Korean - KR (252 KB) Portuguese - PT (252 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.