SSM3 tetraTFA hydrate
Based on 1 Customer Validation
SSM-3 tetraTFA hydrate is a potent furin inhibitor with an EC50 of 54 nM. SSM-3 tetraTFA hydrate uses its positively charged guanidyl group to form strong electrostatic interactions with the negatively charged residues in the catalytic center of furin, thereby competitively occupying and blocking the active site. SSM-3 tetraTFA hydrate can be used in the research of anthrax and avian influenza.
For research use only. We do not sell to patients.
- Purity: 99.9%
- Formula: C30H36F12N12O10.1.5H2O
- Molecular Weight:979.70
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
SSM-3 (1-25 μM; 3 h) partially inhibits the processing of cell surface anthrax toxin protective antigen PA83 into PA63 in glioma U251 cell line[1].
SSM-3 (10-100 μM; 18-24 h) blocks the intracellular processing of pro-MT1-MMP to mature enzyme in the breast cancer MCF7 cell line expressing an MT1-MMP mutant (MCF-MT1-E240A-FLAG cells), and partially inhibits the intracellular processing and release of TGFβ1 in the fibrosarcoma HT1080 cell line[1].
SSM-3 potently inhibits purified human furin, with an EC50 of 54 nM[1].
SSM-3 (0.01-100 μM; 24 h) exhibits cytotoxicity against mouse macrophage RAW 264.7 cells at 100 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human glioma U251 cells
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Concentration:1, 5, 25 μM
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Incubation Time:3 h
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Result:Partially inhibited PA83 processing.
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Cell Line:RAW 264.7 cell line
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Concentration:0.01, 0.1, 1, 10, 100 μM
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Incubation Time:24 h
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Result:Exhibited cell toxicity at the 100 μM concentration.
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Cell Line:HT1080 cell line, MCF7 cell line (MCF-MT1-E240A-FLAG)
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Concentration:10, 100 μM
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Incubation Time:18, 24 h
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Result:Blocked the intracellular processing of MT1-MMP.
Partially suppressed the TGFβ1 processing and release.
Chemical Information
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Appearance Solid
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Molecular Weight 979.70
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Formula C30H36F12N12O10.1.5H2O
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Color White to off-white
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SMILES
N=C(N)NC(C=C1)=CC=C1O[C@@H]2[C@H](C[C@@H](NC(N)=N)[C@H](OC3=CC=C(NC(N)=N)C=C3)C2)NC(N)=N.OC(C(F)(F)F)=O.OC(C(F)(F)F)=O.OC(C(F)(F)F)=O.OC(C(F)(F)F)=O.O.[1.5]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 30 mg/mL (30.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (291 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Remacle AG, et al. Selective and potent furin inhibitors protect cells from anthrax without significant toxicity. The international journal of biochemistry & cell biology. 2010 Jun;42(6):987-95. [Content Brief]
[2]. Jiao GS, et al. Synthetic small molecule furin inhibitors derived from 2,5-dideoxystreptamine. Proceedings of the National Academy of Sciences of the United States of America. 2006 Dec 26;103(52):19707-12. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.0207 mL | 5.1036 mL | 10.2072 mL | 25.5180 mL |
| 5 mM | 0.2041 mL | 1.0207 mL | 2.0414 mL | 5.1036 mL | |
| 10 mM | 0.1021 mL | 0.5104 mL | 1.0207 mL | 2.5518 mL | |
| 15 mM | 0.0680 mL | 0.3402 mL | 0.6805 mL | 1.7012 mL | |
| 20 mM | 0.0510 mL | 0.2552 mL | 0.5104 mL | 1.2759 mL | |
| 25 mM | 0.0408 mL | 0.2041 mL | 0.4083 mL | 1.0207 mL | |
| 30 mM | 0.0340 mL | 0.1701 mL | 0.3402 mL | 0.8506 mL |
SSM3 tetraTFA hydrate Related Classifications
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