Sulfo-SANPAH
Based on 1 Customer Validation
Sulfo-SANPAH is a primary amine-nitrobenzene azide cross-linker. Sulfo-SANPAH improves the functionalization process of PDMS surfaces, is covalently bound to the PAAm gel surface. Sulfo-SANPAH is widely used to crosslink ECM proteins to various substrates, including acrylic-based hydrogels, such as polyacrylamide hydrogels. Sulfo-SANPAH facilitates covalent binding through its negatively charged sulfonate group on its N-hydroxysuccinimide ester ring and a photoactivated phenyl azide group that is highly reactive with nucleophiles and free radicals.
For research use only. We do not sell to patients.
- Purity: 99.75%
- CAS No.: 102568-43-4
- Formula: C16H18N6O9S
- Molecular Weight:470.41
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Sulfo-SANPAH has been widely used to functionalize acrylic-based polymers for ECM attachment[2].
Sulfo-SANPAH improves the functionalization process of PDMS surfaces, including both the time required and the stability of the resulting surface[2].
Sulfo-SANPAH-linked ECM proteins can stabilize cell adhesion to modified PDMS surfaces[2].
Sulfo-SANPAH is covalently bound to the PAAm gel surface and subsequently covalently bound to fibronectin[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 102568-43-4
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Appearance Solid
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Molecular Weight 470.41
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Formula C16H18N6O9S
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Color Orange to red
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SMILES
O=C(ON1C(C(S(=O)(O)=O)CC1=O)=O)CCCCCNC2=CC=C(N=[N+]=[N-])C=C2[N+]([O-])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 125 mg/mL (265.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
1 M NaOH : 25 mg/mL (53.15 mM; ultrasonic and warming and heat to 60°C)
H2O : 14.29 mg/mL (30.38 mM; ultrasonic and warming and adjust pH to 9 with 1 M NaOH and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Vázquez-Victorio G, et al. Fabrication of Adhesive Substrate for Incorporating Hydrogels to Investigate the Influence of Stiffness on Cancer Cell Behavior. Methods Mol Biol. 2021;2174:277-297. [Content Brief]
[2]. Pérez-Calixto, M., et al., (2025). Enhanced PDMS Functionalization for Organ-on-a-Chip Platforms Using Ozone and Sulfo-SANPAH: A Simple Approach for Biomimetic Long-Term Cell Cultures. Advanced healthcare materials, 14(13), e2404686. Advance online publication. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / 1 M NaOH / DMSO | 1 mM | 2.1258 mL | 10.6290 mL | 21.2581 mL | 53.1451 mL |
| 5 mM | 0.4252 mL | 2.1258 mL | 4.2516 mL | 10.6290 mL | |
| 10 mM | 0.2126 mL | 1.0629 mL | 2.1258 mL | 5.3145 mL | |
| 15 mM | 0.1417 mL | 0.7086 mL | 1.4172 mL | 3.5430 mL | |
| 20 mM | 0.1063 mL | 0.5315 mL | 1.0629 mL | 2.6573 mL | |
| 25 mM | 0.0850 mL | 0.4252 mL | 0.8503 mL | 2.1258 mL | |
| 30 mM | 0.0709 mL | 0.3543 mL | 0.7086 mL | 1.7715 mL | |
| 1 M NaOH / DMSO | 40 mM | 0.0531 mL | 0.2657 mL | 0.5315 mL | 1.3286 mL |
| 50 mM | 0.0425 mL | 0.2126 mL | 0.4252 mL | 1.0629 mL | |
| DMSO | 60 mM | 0.0354 mL | 0.1772 mL | 0.3543 mL | 0.8858 mL |
| 80 mM | 0.0266 mL | 0.1329 mL | 0.2657 mL | 0.6643 mL | |
| 100 mM | 0.0213 mL | 0.1063 mL | 0.2126 mL | 0.5315 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Sulfo-SANPAH Related Classifications
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