TAPS 

TAPS is an aminosulfonate-based lysozyme stabilizer and connexin channel inhibitor. TAPS maintains the native structure of lysozyme in aqueous solution at pH 7.0 and significantly protects it from heat-induced denaturation. TAPS directly inhibits the activity of heteromeric connexin 32/connexin 26 channels (Cx32/Cx26) via its protonated form, and this inhibitory effect is dependent on the presence of connexin 26. TAPS reduces connexin channel-mediated solute exchange in a recombinant liposome system, resulting in a decreased degree of liposome density shift in transport-specific separation assays. TAPS is a critical compound for investigating the structure and function of connexin channels^[1][2].

TAPS buffer (0.05-1.0 M) maintains the native structure of chicken egg white lysozyme, as shown by unshifted UV-Vis absorbance peaks^[1].
TAPS buffer (0.05-1.0 M) creates a more stable environment for chicken egg white lysozyme, as shown by a ~7 nm blue shift and decreased fluorescence intensity in Trp-fluorescence spectra^[1].
TAPS buffer (0.05-1.0 M; 2-3 h at 25 °C) maintains the secondary structure of chicken egg white lysozyme, as shown by an unshifted amide I band in FTIR spectra^[1].
TAPS buffer (0.05-1.0 M; 1 h at 25 °C) causes partial loss of secondary structure but enhances the tertiary structure of chicken egg white lysozyme, as measured by CD spectroscopy^[1].
TAPS buffer (0.05-1.0 M; heated from 20 to 85 °C at 5 °C/min) increases the thermal stability of chicken egg white lysozyme in a concentration-dependent manner, raising the melting temperature from 84.8 °C at 0.05 M to 93.7 °C at 1.0 M^[1].
TAPS buffer (0.05-1.0 M) maintains the native conformation of chicken egg white lysozyme, as shown by hydrodynamic diameters staying within 3-5 nm^[1].
TAPS (10 mM; 37 °C) potently inhibits heteromeric Cx32/Cx26 connexin channel activity in a pH-dependent manner, with half-maximum activity at pH 8.5, and does not significantly affect homomeric Cx32 connexin channels^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

243.28

C₇H₁₇NO₆S

29915-38-6

Solid

White to off-white

O=S(CCCNC(CO)(CO)CO)(O)=O

Room temperature in continental US; may vary elsewhere.

Store at room temperature 3 years

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Pannuru P, et al. The effects of biological buffers TRIS, TAPS, TES on the stability of lysozyme. Int J Biol Macromol. 2018;112:720-727.  [Content Brief]

  [2]. Bevans C G, et al. Regulation of connexin channels by pH: direct action of the protonated form of taurine and other aminosulfonates[J]. Journal of Biological Chemistry, 1999, 274(6): 3711-3719.