TBI 

TBI is a fluorescence enhancer with a K_d of 71 nM for the Broccoli fluorogenic RNA aptamer. TBI binds to the Broccoli fluorogenic RNA aptamer to activate its fluorescence.TBI undergoes photobleaching of its trans form, which dissociates rapidly, while cis-TBI from the media replaces the dissociated fluorophore to enable fluorophore recycling.TBI enables enhanced fluorescence of Broccoli during continuous cellular imaging (Ex/Em = 485/527 nm)^[1].

TBI (0.5 μM) binds purified Broccoli RNA to produce a complex with a red-shifted excitation spectrum, high quantum yield, and more than 13,000-fold fluorescence enhancement relative to free TBI^[1].
TBI binds purified Broccoli RNA with high affinity, having a K_d of 71 nM, comparable to BI and significantly higher than DFNS^[1].
TBI (10 μM; 1 h) exhibits low background fluorescence in HEK293T cells and induces high fluorescence specifically in cells expressing the Broccoli RNA aptamer^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

386.38

C₁₈H₁₂F₂N₄O₂S

3053266-55-7

O=C(/C(NC1=S)=C/C2=CC(F)=C(C(F)=C2)O)N1CC3=C4C(N=CN4)=CC=C3

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Li X, et al. Engineering Fluorophore Recycling in a Fluorogenic RNA Aptamer. Angew Chem Int Ed Engl. 2021;60(45):24153-24161.  [Content Brief]