Thermolysin, Bacillus thermoproteolyticus rokko  (Synonyms: EC 3.4.24.27; TML)

Thermolysin, Bacillus thermoproteolyticus rokko (EC 3.4.24.27) (TML) is a thermostable neutral metalloproteinase enzyme secreted by the Gram-positive bacteria Bacillus thermoproteolyticus. Thermolysin catalyzes the hydrolysis of peptide bonds containing hydrophobic residues^[1].

Optimal pH: 8.0. Considerably stable from pH 5 to 9.5.
Optimal temperature : 70 °C

Thermolysin (100 μg in 1 mL, 64°C for 4 h, in isolated starch granules) digestion results in the selective removal of a group of low-molecular-mass proteins ranging between 10 and 27 kD^[2].
Thermolysin can be used for the hydrolysis of bovine liver sarcoplasmic proteins^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Thermolysin (50 mg/kg, p.o., once) lowers systolic blood pressure in SHR rats^[4].
Thermolysin is not acutely toxic with an oral LD₅₀ of more than 18000 mg/kg in rats and more than 24000 mg/kg in mice (Acute toxicity studies)^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9073-78-3

Solid

White to off-white

[Thermolysin]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Bertusvan den Burg, et al. Chapter 111 - Thermolysin and Related Bacillus Metallopeptidases.

  [2]. Mu-Forster C, et al. Surface localization of zein storage proteins in starch granules from maize endosperm. Proteolytic removal by thermolysin and in vitro cross-linking of granule-associated polypeptides. Plant Physiol. 1998 Apr;116(4):1563-71.  [Content Brief]

  [3]. Di Bernardini R, et al. Isolation, purification and characterization of antioxidant peptidic fractions from a bovine liver sarcoplasmic protein thermolysin hydrolyzate. Peptides. 2011 Feb;32(2):388-400.  [Content Brief]

  [4]. Fujita H, et al: a prodrug-type ACE-inhibitory peptide derived from fish protein. Immunopharmacology. 1999 Oct 15;44(1-2):123-7.  [Content Brief]

  [5]. Ke Q, et al. Safety evaluation of a thermolysin enzyme produced from Geobacillus stearothermophilus. Food Chem Toxicol. 2013 Sep;59:541-8.  [Content Brief]